{
  "abstract": "Spatial analysis of cells and their microenvironment within tissues enhances our understanding of biological processes. Ideally, a broad range of biomolecules should be analyzed in large 3D tissue specimens at subcellular resolution. Here, we present a protocol to identify and extract target sections from previously cleared tissues. We describe steps for combining 3D light sheet imaging and sub- sequent 3D-guided deep cell phenotyping via multi-cyclic 2D microscopy",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 11,
  "authors": [
    {
      "name": "Kevin Bigott"
    },
    {
      "name": "Victoria H Schoppel"
    },
    {
      "name": "Manuel Martinez-Osuna"
    },
    {
      "name": "Leon Osinski"
    },
    {
      "name": "Marie-Catherine Tiveron"
    },
    {
      "name": "Daniel Barleben"
    },
    {
      "name": "Simon F Bornemann"
    },
    {
      "name": "Harold Cremer"
    },
    {
      "name": "Christoph Herbel"
    },
    {
      "name": "Andreas Bosio"
    },
    {
      "name": "Melanie Jungblut"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/01ds3tct0sy4n.bib",
  "cited_by": 3,
  "doi": "10.1016/j.xpro.2025.104296",
  "fulltext_url": null,
  "id": "01ds3tct0sy4n",
  "issns": [
    "2666-1667"
  ],
  "landing_url": "https://hal.science/hal-05795744v1",
  "language": "en",
  "licence": "CC-BY-NC-ND-4.0",
  "licence_label": "CC BY-NC-ND 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "hal",
      "0.sdv"
    ],
    [
      "openalex",
      "1304|Cell Image Analysis Techniques"
    ]
  ],
  "openalex": "W7117374000",
  "pdf_url": "https://hal.science/hal-05795744/document",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-12",
  "publisher": "Elsevier",
  "ris": "https://research.draconitic.com/paper/01ds3tct0sy4n.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:55Z",
      "native_id": "hal-05795744",
      "source": "hal",
      "url": "https://hal.science/hal-05795744v1"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1304|Cell Image Analysis Techniques",
      "subfield": "biophysics"
    }
  ],
  "title": "Protocol for 3D-guided sectioning and deep cell phenotyping via light sheet imaging and 2D spatial multiplexing",
  "title_text": "Protocol for 3D-guided sectioning and deep cell phenotyping via light sheet imaging and 2D spatial multiplexing",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:55Z",
  "url": "https://research.draconitic.com/paper/01ds3tct0sy4n",
  "venue": "STAR Protocols",
  "versions": []
}