{
  "abstract": "Light-sheet fluorescence microscopy (LSFM) enables gentle, rapid and efficient volumetric imaging of biological specimens. Despite its potential, LSFM has fragmented into multiple variants, each narrowly optimized for specific samples or imaging regimes, limiting its broad applicability. Here we introduce the multi-immersion oblique plane microscope (miOPM), an adaptable LSFM platform for high-resolution imaging ranging from subcellular dynamics to whole organisms and cleared tissues. miOPM supports seamless interchangeability of oil, water and air objectives and maintains diffraction-limited performance across a refractive index range of 1.33 to 1.51. It is compatible with standard sample mounting, current clearing protocols and high-throughput 3D imaging. We leverage miOPM to image diverse biological specimens at multiple spatial scales. By expanding the adaptability and ease of use of LSFM, miOPM is poised to democratize advanced 3D imaging.",
  "abstract_shown": true,
  "affiliations": [
    "Lyda Hill Department of Bioinformatics, UT Southwestern Medical Center, Dallas, TX USA",
    "Cecil H. and Ida Green Center for Systems Biology, UT Southwestern Medical Center, Dallas, TX USA",
    "Calico Life Sciences LLC, South San Francisco, CA USA",
    "Department of Systems Biology and Engineering, Silesian University of Technology, Gliwice, Poland",
    "Center for Alzheimer’s and Neurodegenerative Diseases, Peter O’Donnell Jr. Brain Institute, UT Southwestern Medical Center, Dallas, TX USA",
    "Present Address: Ubiquitin Signalling Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, Australia",
    "Present Address: CSSB Centre for Structural Systems Biology, Deutsches Elektronen-Synchrotron DESY, Hamburg, Germany",
    "Kennedy Institute of Rheumatology, Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences (NDORMS), University of Oxford, Oxford, UK",
    "Department of Cell Biology, UT Southwestern Medical Center, Dallas, TX USA",
    "Department of Biochemistry & Biophysics, University of California, San Francisco, San Francisco, CA USA",
    "Department of Biological Science, Columbia University, New York, NY USA",
    "Present Address: Princeton Neuroscience Institute, Princeton University, Princeton, NJ USA",
    "Department of Biophysics, UT Southwestern Medical Center, Dallas, TX USA"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 27,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Bingying Chen"
    },
    {
      "affs": [
        2
      ],
      "name": "Alfred Millett-Sikking"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Bo-Jui Chang",
      "orcid": "0000-0002-5513-7106"
    },
    {
      "affs": [
        0,
        1,
        3
      ],
      "name": "Seweryn Gałecki",
      "orcid": "0000-0002-7020-0804"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Stephan Daetwyler",
      "orcid": "0000-0002-7444-4734"
    },
    {
      "affs": [
        4,
        5
      ],
      "name": "Jenny Jiou"
    },
    {
      "affs": [
        4,
        6
      ],
      "name": "Jim Monistrol"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Qionghua Shen",
      "orcid": "0000-0002-2808-4408"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Felix Zhou",
      "orcid": "0000-0003-4463-1165"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Hsin-Yu Lin"
    },
    {
      "affs": [
        7
      ],
      "name": "Edward Jenkins",
      "orcid": "0000-0003-3789-6421"
    },
    {
      "affs": [
        8
      ],
      "name": "Miriam C. Stein"
    },
    {
      "affs": [
        8
      ],
      "name": "Madeleine Marlar-Pavey"
    },
    {
      "affs": [
        2,
        9
      ],
      "name": "Gabriel Sturm"
    },
    {
      "affs": [
        2
      ],
      "name": "Alexander L. Li",
      "orcid": "0000-0002-1233-0950"
    },
    {
      "affs": [
        2
      ],
      "name": "Qiaosi Tang"
    },
    {
      "affs": [
        2
      ],
      "name": "Brian Y. Feng",
      "orcid": "0000-0002-4208-8624"
    },
    {
      "affs": [
        9
      ],
      "name": "Ulises Diaz"
    },
    {
      "affs": [
        10,
        11
      ],
      "name": "Yannan Chen",
      "orcid": "0000-0002-2959-2885"
    },
    {
      "affs": [
        2
      ],
      "name": "Aryaman Shalizi"
    },
    {
      "affs": [
        2
      ],
      "name": "Astrid Gillich"
    },
    {
      "affs": [
        8
      ],
      "name": "Jonathan R. Friedman",
      "orcid": "0000-0001-8155-2429"
    },
    {
      "affs": [
        10
      ],
      "name": "Raju Tomer",
      "orcid": "0000-0002-4229-2860"
    },
    {
      "affs": [
        9
      ],
      "name": "Wallace F. Marshall"
    },
    {
      "affs": [
        4,
        12
      ],
      "name": "Sarah Shahmoradian"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Kevin M. Dean",
      "orcid": "0000-0003-0839-2320"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Reto Fiolka",
      "orcid": "0000-0002-4636-5000"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/0295jdc00s9m6.bib",
  "cited_by": 2,
  "doi": "10.1038/s41592-026-03245-0",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13736197/",
  "id": "0295jdc00s9m6",
  "issns": [
    "1548-7091",
    "1548-7105"
  ],
  "landing_url": "https://doi.org/10.1038/s41592-026-03245-0",
  "language": "en",
  "licence": "CC-BY-NC-ND-4.0",
  "licence_label": "CC BY-NC-ND 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Article"
    ],
    [
      "openalex",
      "1304|Advanced Fluorescence Microscopy Techniques"
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  "openalex": "W7222930764",
  "pdf_url": "https://www.nature.com/articles/s41592-026-03245-0.pdf",
  "pmcid": "PMC13736197",
  "pmid": "42897240",
  "published": "2026-10-19",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/0295jdc00s9m6.ris",
  "sources": [
    {
      "first_seen": "2026-11-09T12:21:42Z",
      "native_id": "PMC13736197",
      "source": "pmc",
      "url": "https://doi.org/10.1038/s41592-026-03245-0"
    }
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  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1304|Advanced Fluorescence Microscopy Techniques",
      "subfield": "biophysics"
    }
  ],
  "title": "Multi-immersion oblique plane microscope (miOPM): a reconfigurable platform for high-resolution light-sheet fluorescence microscopy",
  "title_text": "Multi-immersion oblique plane microscope (miOPM): a reconfigurable platform for high-resolution light-sheet fluorescence microscopy",
  "type": "peer-reviewed",
  "updated_at": "2026-11-09T12:21:42Z",
  "url": "https://research.draconitic.com/paper/0295jdc00s9m6",
  "venue": "Nature Methods",
  "versions": []
}