{
  "abstract": "In this study, we propose a facile and efficient method for constructing artificial multicellular models via vibration-induced flow (VIF), a local fluid flow induced around vibrating microstructures. By applying circular vibration to a substrate with a cylindrical micropillar array, uniform micro-vortices were generated, into which giant unilamellar vesicles (GUVs), as artificial cells, rapidly accumulated at the center. We show that by modifying two GUV populations with complementary single-stranded DNAs, we can promote specific adhesion between GUVs, leading to uniform parallel aggregates. Furthermore, the pillar-array design controls the size of GUV aggregates. Finally, we embedded nanopore-forming α-hemolysin with Spytag and SpyCatcher system in GUV populations, allowing the lumens of assembled GUVs to channel with one another and enable close-proximity molecular communication. This technology will contribute to future research on fabricating artificial multicellular models with complex functions.",
  "abstract_shown": true,
  "affiliations": [
    "Graduate School of Science and Engineering, Chuo University, 1-13-27 Kasuga, Bunkyo-ku, Tokyo, Japan, 112-8551",
    "Earth-Life Science Institute (ELSI, Institute of Science Tokyo, 2-12-1 Ookayama, Meguro-ku, Tokyo, Japan, 152-8550"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Daiki Miyashita"
    },
    {
      "affs": [
        0
      ],
      "name": "Yui Katsumata"
    },
    {
      "affs": [
        1
      ],
      "name": "Xu Han"
    },
    {
      "affs": [
        0
      ],
      "name": "Zhitai Huang"
    },
    {
      "affs": [
        0
      ],
      "name": "Mamiko Tsugane"
    },
    {
      "affs": [
        1
      ],
      "name": "Tomoaki Matsuura"
    },
    {
      "affs": [
        0
      ],
      "name": "Hiroaki Suzuki",
      "orcid": "0000-0002-8899-0955"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/02bv7jk5ecwgr.bib",
  "cited_by": 0,
  "doi": "10.26434/chemrxiv.15010019/v1",
  "fulltext_url": null,
  "id": "02bv7jk5ecwgr",
  "issns": [],
  "landing_url": "https://chemrxiv.org/doi/full/10.26434/chemrxiv.15010019/v1",
  "language": null,
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Lipid Membrane Structure and Behavior"
    ]
  ],
  "openalex": "W7220850478",
  "pdf_url": "https://chemrxiv.org/doi/pdf/10.26434/chemrxiv.15010019/v1",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-07",
  "publisher": "American Chemical Society (ACS)",
  "ris": "https://research.draconitic.com/paper/02bv7jk5ecwgr.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:22Z",
      "native_id": "10.26434/chemrxiv.15010019",
      "source": "chemrxiv",
      "url": "https://chemrxiv.org/doi/full/10.26434/chemrxiv.15010019/v1"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Lipid Membrane Structure and Behavior",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Parallel Production of Uniform Artificial Multicellular Models Using Vibration-Induced Local Microvortices",
  "title_text": "Parallel Production of Uniform Artificial Multicellular Models Using Vibration-Induced Local Microvortices",
  "type": "preprint",
  "updated_at": "2026-10-30T12:17:22Z",
  "url": "https://research.draconitic.com/paper/02bv7jk5ecwgr",
  "venue": "ChemRxiv",
  "versions": []
}