{
  "abstract": "Pseudomonas aeruginosa (P.a) is a Gram-negative opportunistic pathogen that poses a major global health threat, particularly in immunocompromised individuals, patients with cystic fibrosis, and those with burn injuries or ventilator-associated pneumonia. Despite intense efforts, no vaccine is available for human use. In this context, live attenuated vaccines represent a promising but underexplored approach, offering the potential to elicit robust, long-lasting, and multifaceted immune responses including inducing trained immunity. Here, we sub-cultured ΔLasB-PAO1 (a P.a strain we have previously shown to have reduced virulence) in artificial sputum medium, a culture medium mimicking cystic fibrosis (CF) sputum in which bacteria often show auxotrophy. We showed that such strain (\"V\" for vaccine) was auxotrophic, less virulent, and had characteristics of \"CF-like strains.\" Crucially, \"V\" induced both local (IgA) and systemic humoral responses as well as memory Th17 immune responses, and could, when administered in the lung, but not intra-muscularly, protect mice against a lethal PAO1-WT infection. Overall, the present study demonstrates that our vaccine formulation, in addition to providing an advantageous auxotrophic phenotype adapted to the CF setting, was efficient, when given mucosally and was associated with a pathway involving Th17 responses and secretory IgA, a critical barrier that neutralizes pathogens before tissue invasion.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 12,
  "authors": [
    {
      "name": "Youssouf Sereme"
    },
    {
      "name": "Bérengère Villeret"
    },
    {
      "name": "Ségolène Caboche"
    },
    {
      "name": "Samuel Ikeh"
    },
    {
      "name": "Hugo Leroy"
    },
    {
      "name": "Delphine Beury"
    },
    {
      "name": "Florence Maurier"
    },
    {
      "name": "Christophe Desterke"
    },
    {
      "name": "Maëlys Born-Bony"
    },
    {
      "name": "Zhou Xing"
    },
    {
      "name": "Romé Voulhoux"
    },
    {
      "name": "Jean-Michel Sallenave"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/038y0hgh6q01f.bib",
  "cited_by": 1,
  "doi": "10.1016/j.ymthe.2026.06.029",
  "fulltext_url": null,
  "id": "038y0hgh6q01f",
  "issns": [
    "1525-0016",
    "1525-0024"
  ],
  "landing_url": "https://amu.hal.science/hal-05767654v1",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "hal",
      "0.sdv"
    ],
    [
      "openalex",
      "1312|Bacterial biofilms and quorum sensing"
    ]
  ],
  "openalex": "W7165045544",
  "pdf_url": "https://amu.hal.science/hal-05767654/document",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-28",
  "publisher": "Cell Press",
  "ris": "https://research.draconitic.com/paper/038y0hgh6q01f.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:42Z",
      "native_id": "hal-05767654",
      "source": "hal",
      "url": "https://amu.hal.science/hal-05767654v1"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Bacterial biofilms and quorum sensing",
      "subfield": "molecular-biology"
    }
  ],
  "title": "A novel cystic fibrosis-mimetic Pseudomonas auxotrophic vaccine is protective in vivo and is associated with IL-17/IgA mucosal Immunity",
  "title_text": "A novel cystic fibrosis-mimetic Pseudomonas auxotrophic vaccine is protective in vivo and is associated with IL-17/IgA mucosal Immunity",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:42Z",
  "url": "https://research.draconitic.com/paper/038y0hgh6q01f",
  "venue": "Molecular Therapy",
  "versions": []
}