{
  "abstract": "Objective: Enfortumab vedotin (EV) and cisplatin (CDDP) are central treatments for advanced urothelial carcinoma, yet the influence of prior CDDP exposure on subsequent EV efficacy remains unclear. In this report, we characterized EV-related drug responses in a CDDP-resistant bladder cancer subline. Methods: A CDDP-resistant subline (HT1376-CR) was generated from parental bladder cancer cell (HT1376/P) through continuous exposure to CDDP. Sensitivities to CDDP, EV, and the EV payload monomethyl auristatin E (MMAE) were compared between HT1376-CR and HT1376/P. Nectin-4 expression, EV binding, and drug-resistance-related gene expression were evaluated. The effect of ABCB1 silencing on drug response was also examined. In vivo EV responses were explored using xenograft models. Results: HT1376-CR cells showed increased sensitivity to EV in vitro and in vivo. HT1376-CR cells were more sensitive to MMAE and showed greater G2/M accumulation after MMAE exposure. In contrast, cell-surface nectin-4 expression and EV binding were reduced. ABCB1 was markedly upregulated in HT1376-CR, and ABCB1 silencing increased sensitivity to CDDP, MMAE, and EV within the resistant subline. Conclusion: The CDDP-resistant HT1376 subline showed enhanced sensitivity to EV, and ABCB1 suppression further increased EV sensitivity within this resistant subline.",
  "abstract_shown": true,
  "affiliations": [
    "Division of Urology, Department of Surgery Related, Kobe University Graduate School of Medicine, Japan."
  ],
  "article_type": "Research Article",
  "arxiv": null,
  "author_total": 13,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Taisuke Tobe",
      "orcid": "0000-0001-9289-9014"
    },
    {
      "affs": [
        0
      ],
      "name": "Jun Teishima",
      "orcid": "0009-0004-7018-5095"
    },
    {
      "affs": [
        0
      ],
      "name": "Takuto Hara"
    },
    {
      "affs": [
        0
      ],
      "name": "Yusuke Shiraishi",
      "orcid": "0009-0000-3938-5719"
    },
    {
      "affs": [
        0
      ],
      "name": "Hideto Ueki"
    },
    {
      "affs": [
        0
      ],
      "name": "Naoto Wakita",
      "orcid": "0009-0007-1353-7789"
    },
    {
      "affs": [
        0
      ],
      "name": "Yasuyoshi Okamura"
    },
    {
      "affs": [
        0
      ],
      "name": "Yukari Bando"
    },
    {
      "affs": [
        0
      ],
      "name": "Kotaro Suzuki"
    },
    {
      "affs": [
        0
      ],
      "name": "Tomoaki Terakawa"
    },
    {
      "affs": [
        0
      ],
      "name": "Akihisa Yao"
    },
    {
      "affs": [
        0
      ],
      "name": "Koji Chiba"
    },
    {
      "affs": [
        0
      ],
      "name": "Hideaki Miyake"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/03a4vpg0m4sc7.bib",
  "cited_by": 0,
  "doi": "10.1371/journal.pone.0361593",
  "fulltext_url": "https://europepmc.org/articles/PMC13718962",
  "id": "03a4vpg0m4sc7",
  "issns": [
    "1932-6203"
  ],
  "landing_url": "https://doi.org/10.1371/journal.pone.0361593",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Biochemistry"
    ],
    [
      "heading",
      "Biology and Life Sciences"
    ],
    [
      "heading",
      "Biology and life sciences"
    ],
    [
      "heading",
      "Bladder Cancer"
    ],
    [
      "heading",
      "Cancer Treatment"
    ],
    [
      "heading",
      "Cancers and Neoplasms"
    ],
    [
      "heading",
      "Cell Binding"
    ],
    [
      "heading",
      "Cell Biology"
    ],
    [
      "heading",
      "Cell Cycle and Cell Division"
    ],
    [
      "heading",
      "Cell Physiology"
    ],
    [
      "heading",
      "Cell Processes"
    ],
    [
      "heading",
      "Cytophotometry"
    ],
    [
      "heading",
      "Flow Cytometry"
    ],
    [
      "heading",
      "Gene expression"
    ],
    [
      "heading",
      "Gene regulation"
    ],
    [
      "heading",
      "Genetics"
    ],
    [
      "heading",
      "Genitourinary Cancers"
    ],
    [
      "heading",
      "Genitourinary Tract Tumors"
    ],
    [
      "heading",
      "Malignant Tumors"
    ],
    [
      "heading",
      "Medicine and Health Sciences"
    ],
    [
      "heading",
      "Non-coding RNA"
    ],
    [
      "heading",
      "Nucleic acids"
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    ],
    [
      "heading",
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    ],
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      "heading",
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    ],
    [
      "heading",
      "Research and Analysis Methods"
    ],
    [
      "heading",
      "Small interfering RNA"
    ],
    [
      "heading",
      "Spectrophotometry"
    ],
    [
      "heading",
      "Spectrum Analysis Techniques"
    ],
    [
      "heading",
      "Urology"
    ],
    [
      "plos",
      "/Biology and life sciences/Biochemistry/Nucleic acids/RNA/Non-coding RNA/Small interfering RNA"
    ],
    [
      "plos",
      "/Biology and life sciences/Cell biology/Cell physiology/Cell binding"
    ],
    [
      "plos",
      "/Biology and life sciences/Cell biology/Cell processes/Cell cycle and cell division"
    ],
    [
      "plos",
      "/Biology and life sciences/Genetics/Gene expression/Gene regulation/Small interfering RNA"
    ],
    [
      "plos",
      "/Medicine and health sciences/Oncology/Cancer treatment"
    ],
    [
      "plos",
      "/Medicine and health sciences/Oncology/Cancers and neoplasms"
    ],
    [
      "plos",
      "/Medicine and health sciences/Oncology/Cancers and neoplasms/Genitourinary tract tumors/Bladder cancer"
    ],
    [
      "plos",
      "/Medicine and health sciences/Oncology/Cancers and neoplasms/Malignant tumors"
    ],
    [
      "plos",
      "/Medicine and health sciences/Urology/Genitourinary cancers/Bladder cancer"
    ],
    [
      "plos",
      "/Research and analysis methods/Spectrum analysis techniques/Spectrophotometry/Cytophotometry/Flow cytometry"
    ]
  ],
  "openalex": null,
  "pdf_url": "https://europepmc.org/articles/PMC13718962?pdf=render",
  "pmcid": "PMC13718962",
  "pmid": "42955934",
  "published": "2026-10-29",
  "publisher": "Public Library of Science",
  "ris": "https://research.draconitic.com/paper/03a4vpg0m4sc7.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:17Z",
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  "subjects": [
    {
      "field": "biochemistry",
      "origin": "heading:Gene expression",
      "subfield": "molecular-biology"
    },
    {
      "field": "biology",
      "origin": "plos:cell biology",
      "subfield": "cell-biology"
    },
    {
      "field": "biology",
      "origin": "heading:Genetics",
      "subfield": "genetics-genomics"
    },
    {
      "field": "medicine",
      "origin": "plos:oncology",
      "subfield": "oncology"
    }
  ],
  "title": "Enhanced enfortumab vedotin sensitivity in a cisplatin-resistant bladder cancer model",
  "title_text": "Enhanced enfortumab vedotin sensitivity in a cisplatin-resistant bladder cancer model",
  "type": "peer-reviewed",
  "updated_at": "2026-11-02T12:22:53Z",
  "url": "https://research.draconitic.com/paper/03a4vpg0m4sc7",
  "venue": "PLOS One",
  "versions": []
}