{
  "abstract": "Polysaccharides have attracted extensive attention due to their pharmacological activities, such as regulating depression, antitumor, hypoglycemic and lipid-lowering effects, and regulating intestinal flora. In this study, four crude polysaccharide fractions, designated LICP007, LICP008, LICP009 and LICP010, were prepared from Lycium barbarum L. Based on the in vitro α-amylase and α-glucosidase inhibitory assays, LICP007, which showed the strongest activity among the four crude polysaccharides (inhibition rates of 61.42 ± 0.29% against α-amylase and 40.69 ± 0.35% against α-glucosidase at 50 mg/mL), was selected for further separation and purification, and one neutral polysaccharide (LICP007-N1) and two acidic polysaccharides (LICP007-S2 and LICP007-S5) were obtained by DEAE-52 cellulose anion-exchange and Sephadex G-200 gel permeation chromatography. Among them, LICP007-S5 showed the best inhibitory activity against α-amylase and α-glucosidase, with inhibition rates of 98.38% and 43.45% at 20 mg/mL, respectively. Physicochemical characterization showed that LICP007-S5 differed markedly from LICP007-N1 and LICP007-S2 in molecular weight distribution, monosaccharide composition and morphology. Structural analysis indicated that LICP007-S5 is an acidic heteropolysaccharide rich in galacturonic acid (27.5 mol%); atomic force microscopy (AFM) and Congo red test results suggest that it may adopt an ordered, possibly triple-helical conformation, although this requires further verification. These findings provide comparative information on the structure and biological activity of different Lycium barbarum polysaccharides and highlight their potential as natural α-amylase and α-glucosidase inhibitors for the dietary management of type 2 diabetes in functional foods.",
  "abstract_shown": true,
  "affiliations": [
    "National Wolfberry Engineering Research Center, Wolfberry Science Research Institute, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 750002, China",
    "School of Pharmacy, Gansu University of Chinese Medicine, Lanzhou 730000, China"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Xuan Zhou"
    },
    {
      "affs": [
        1
      ],
      "name": "Yanyan Liu"
    },
    {
      "affs": [
        0
      ],
      "name": "Ting Huang"
    },
    {
      "affs": [
        0
      ],
      "name": "Linyuan Duan"
    },
    {
      "affs": [
        0
      ],
      "name": "Yuekun Li"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/03hq49a30jj7x.bib",
  "cited_by": 0,
  "doi": "10.3390/molecules31213839",
  "fulltext_url": null,
  "id": "03hq49a30jj7x",
  "issns": [
    "1420-3049"
  ],
  "landing_url": "https://www.mdpi.com/1420-3049/31/21/3839",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1106|Polysaccharides Composition and Applications"
    ]
  ],
  "openalex": "W7223664599",
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-23",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/03hq49a30jj7x.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:42Z",
      "native_id": "10.3390/molecules31213839",
      "source": "crossref",
      "url": "https://www.mdpi.com/1420-3049/31/21/3839"
    }
  ],
  "subjects": [
    {
      "field": "chemistry",
      "origin": "issn:1420-3049",
      "subfield": "general"
    }
  ],
  "title": "Fractionation, Structural Characterization and In Vitro α-Amylase and α-Glucosidase Inhibitory Activities of Polysaccharides Derived from Lycium barbarum L.",
  "title_text": "Fractionation, Structural Characterization and In Vitro α-Amylase and α-Glucosidase Inhibitory Activities of Polysaccharides Derived from Lycium barbarum L.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:18:42Z",
  "url": "https://research.draconitic.com/paper/03hq49a30jj7x",
  "venue": "Molecules",
  "versions": []
}