{
  "abstract": "Cilia possess the inherent ability to restrict the entry of non-ciliary components through the ciliary gate. Mutations in genes encoding components of the ciliary gate result in various cilia-related diseases known as ciliopathies. Yet, how non-ciliary residents are prevented from accumulating in cilia remains unclear. Here, we observe ciliary accumulation of non-ciliary proteins in BBSome-deficient mutants (Bardet-Biedl syndrome), a phenotype reminiscent of ciliary gate-defective mutants such as mks-5 and nphp-4 . Time-lapse imaging shows that these non-ciliary proteins move bidirectionally along cilia at IFT velocities, and bimolecular fluorescence complementation (BiFC) reveals that the non-ciliary protein ELMD-1 associates with the IFT component IFT-43 in BBSome-deficient mutants, indicating that they are mis-loaded onto IFT trains. This misloading allows them to bypass the ciliary gate. Fluorescence recovery after photobleaching (FRAP) suggests the gate remains largely functional, supporting a potential two-step model of ciliary composition control: cargo selection/loading and ciliary gating. We propose that defects in cargo selection/loading (and potentially export) alter ciliary composition, allowing unintended protein entry.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 11,
  "authors": [
    {
      "name": "Sebiha Cevik",
      "orcid": "0000-0002-0935-1929"
    },
    {
      "name": "Ferhan Yenisert"
    },
    {
      "name": "Hanife Kantarci",
      "orcid": "0000-0003-4322-7101"
    },
    {
      "name": "Damla Pucak"
    },
    {
      "name": "Christian Gallrein"
    },
    {
      "name": "Simon Stöckl"
    },
    {
      "name": "Xenia Dünninger"
    },
    {
      "name": "Onur Cakici",
      "orcid": "0000-0002-6809-4467"
    },
    {
      "name": "Christian Stigloher"
    },
    {
      "name": "Janine Kirstein",
      "orcid": "0000-0003-4990-2497"
    },
    {
      "name": "Oktay I. Kaplan",
      "orcid": "0000-0002-8733-0920"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/03qzwze4s51gy.bib",
  "cited_by": 0,
  "doi": "10.1038/s42003-026-10986-3",
  "fulltext_url": null,
  "id": "03qzwze4s51gy",
  "issns": [
    "2399-3642"
  ],
  "landing_url": "https://www.nature.com/articles/s42003-026-10986-3",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1311|Genetic and Kidney Cyst Diseases"
    ]
  ],
  "openalex": "W7223555405",
  "pdf_url": "https://www.nature.com/articles/s42003-026-10986-3_reference.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-25",
  "publisher": "Springer Science and Business Media LLC",
  "ris": "https://research.draconitic.com/paper/03qzwze4s51gy.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:50Z",
      "native_id": "10.1038/s42003-026-10986-3",
      "source": "crossref",
      "url": "https://www.nature.com/articles/s42003-026-10986-3"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1311|Genetic and Kidney Cyst Diseases",
      "subfield": "genetics-genomics"
    }
  ],
  "title": "A role for the BBSome in restricting the entry of non-ciliary proteins into cilia",
  "title_text": "A role for the BBSome in restricting the entry of non-ciliary proteins into cilia",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:50Z",
  "url": "https://research.draconitic.com/paper/03qzwze4s51gy",
  "venue": "Communications Biology",
  "versions": []
}