{
  "abstract": "Introduction Rheumatoid arthritis (RA) is a chronic autoimmune disease characterised by synovial inflammation and osteoclast-driven bone erosion, leading to joint destruction. T cells play a central role in driving inflammation and contribute to the pathogenesis of RA. This study aims to identify T cell subsets in RA using single-cell RNA sequencing (scRNA-seq) technology.Methods An in silico analysis of publicly available scRNA-seq datasets was conducted to identify a T cell population enriched in RA synovial tissue. To generate this population in vitro, peripheral blood T cells were stimulated with various cytokines. Gene expression was measured by quantitative PCR, and bulk RNA sequencing was used to define the transcriptomic profile of this population. Proteomic analysis was also performed to characterise its protein expression profile and functional pathways.Results A population of T cells expressing immunoglobulin genes was identified in RA synovial tissue in silico. Among the various cytokines tested, interleukin-4 specifically upregulated immunoglobulin gene expression in peripheral blood lymphocytes, particularly in naive CD8+ T cells. Transcriptomic analysis showed increased osteoclast differentiation but reduced pathways related to osteoclast differentiation regulation, RA and inflammatory responses. In contrast, proteomic analysis showed consistent downregulation of the osteoclast differentiation and inflammatory response pathways.Conclusions These findings suggest a T cell population in RA exhibiting immunoglobulin gene expression at the transcript level and reduced inflammatory and osteoclast-related pathways at the protein level.",
  "abstract_shown": true,
  "affiliations": [
    "The First Department of Internal Medicine, School of Medicine, University of Occupational and Environmental Health Japan, Kitakyushu, Japan",
    "Department of Clinical Nursing, School of Health Sciences, University of Occupational and Environmental Health Japan, Kitakyushu, Japan",
    "Research Unit, Research Division, Tanabe Pharma Corp, Yokohama, Japan",
    "Department of Immunology and Parasitology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan",
    "Faculty of Public Health, University of Medicine and Pharmacy, Hue University, Hue, Viet Nam",
    "Graduate School of Life Sciences and Biotechnology, Medical Creation University, Iwaki, Japan",
    "Department of Molecular Targeted Therapeutics, University of Occupational and Environmental Health, Kitakyushu, Japan"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 12,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Tuan Manh Nguyen",
      "orcid": "0009-0005-5022-9799"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Koshiro Sonomoto",
      "orcid": "0000-0002-4003-8842"
    },
    {
      "affs": [
        0
      ],
      "name": "Kaoru Yamagata"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Junpei Anan"
    },
    {
      "affs": [
        0
      ],
      "name": "Yuya Fujita",
      "orcid": "0000-0003-4163-9356"
    },
    {
      "affs": [
        0,
        3
      ],
      "name": "Zirui Zeng"
    },
    {
      "affs": [
        0,
        4
      ],
      "name": "Ngo Thi Dieu Huong",
      "orcid": "0000-0003-4920-4509"
    },
    {
      "affs": [
        0
      ],
      "name": "Ngo Thi Uyen"
    },
    {
      "affs": [
        0
      ],
      "name": "Le Thi Huyen Trang"
    },
    {
      "affs": [
        5
      ],
      "name": "Shigeaki Kato"
    },
    {
      "affs": [
        0,
        6
      ],
      "name": "Yoshiya Tanaka",
      "orcid": "0000-0002-0807-7139"
    },
    {
      "affs": [
        0
      ],
      "name": "Shingo Nakayamada",
      "orcid": "0000-0002-2209-8454"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/052v6zm0qkd3z.bib",
  "cited_by": 0,
  "doi": "10.1136/rmdopen-2026-006988",
  "fulltext_url": "https://rmdopen.bmj.com/content/12/4/e006988.full",
  "id": "052v6zm0qkd3z",
  "issns": [
    "2056-5933"
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  "landing_url": "https://doi.org/10.1136/rmdopen-2026-006988",
  "language": "en",
  "licence": "CC-BY-NC-4.0",
  "licence_label": "CC BY-NC 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "1506"
    ],
    [
      "heading",
      "Original Research"
    ],
    [
      "heading",
      "Rheumatoid Arthritis"
    ],
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      "lcc",
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      "openalex",
      "2745|Rheumatoid Arthritis Research and Therapies"
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  "pdf_url": "https://rmdopen.bmj.com/content/12/4/e006988.full.pdf",
  "pmcid": "PMC13711577",
  "pmid": "42919622",
  "published": "2026-10-23",
  "publisher": "BMJ Publishing Group",
  "ris": "https://research.draconitic.com/paper/052v6zm0qkd3z.ris",
  "sources": [
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      "first_seen": "2026-10-30T13:44:10Z",
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      "origin": "openalex:2745|Rheumatoid Arthritis Research and Therapies",
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  "title": "Interleukin-4-induced transcription of immunoglobulin genes in CD8+ T cells: implications for rheumatoid arthritis",
  "title_text": "Interleukin-4-induced transcription of immunoglobulin genes in CD8+ T cells: implications for rheumatoid arthritis",
  "type": "peer-reviewed",
  "updated_at": "2026-10-31T12:22:20Z",
  "url": "https://research.draconitic.com/paper/052v6zm0qkd3z",
  "venue": "RMD Open",
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}