{
  "abstract": "Intramuscular fat (IMF) content is a key determinant of pork quality. In this study, the Chinese indigenous Huai pig (a fat-type breed) and the Western Duroc pig (a lean-type breed) were selected to investigate the molecular mechanisms underlying IMF deposition. Proteomic analysis identified 91 differentially expressed proteins (DEPs) between the two breeds. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed that several key lipid metabolism-related pathways were significantly enriched, including the Lipid metabolic process, AMPK signaling pathway, nicotinate and nicotinamide metabolism, and Fat digestion and absorption. Western blotting further confirmed the reliability of the proteomic results. Integrated transcriptomic and proteomic analyses highlighted significant enrichment of the PPAR signaling pathway, which plays a central role in lipid metabolism. These findings suggest that enhanced fatty-acid uptake contributes to increased lipid deposition in muscle tissue. Additionally, several potential candidate genes associated with IMF deposition were identified, including CD36, SCARB2, ACADVL, ACADSB, ACADM, UNC119B, PAS-4, and APOA1. Collectively, this study provides valuable insights into the molecular regulation of IMF deposition in pigs and offers a theoretical basis and genetic resources for improving pork quality and advancing molecular breeding.",
  "abstract_shown": true,
  "affiliations": [
    "College of Animal Science, Anhui Science and Technology University, Chuzhou, China",
    "Anhui Province Key Laboratory of Animal Nutritional Regulation and Health, Chuzhou, China",
    "Anhui Engineering Technology Research Center of Pork Quality Control and Enhance, Chuzhou, China",
    "National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China",
    "Desert Research Center, Cairo, Egypt",
    "Institute of Animal Husbandry and Veterinary Medicine, Anhui Academy of Agricultural Sciences, Hefei, China"
  ],
  "article_type": "Research Article",
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Caoyan Xu"
    },
    {
      "affs": [
        1
      ],
      "name": "Pengcheng Shen"
    },
    {
      "affs": [
        2
      ],
      "name": "Chang Sheng Jiang"
    },
    {
      "affs": [
        3
      ],
      "name": "Ahmed H. Ghonaim"
    },
    {
      "affs": [
        4
      ],
      "name": "Man Ren"
    },
    {
      "affs": [
        5
      ],
      "name": "Xiaojin Li"
    },
    {
      "name": "Qianqian Hu"
    },
    {
      "name": "Chonglong Wang"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/05pwfpn58sc0a.bib",
  "cited_by": 0,
  "doi": "10.1371/journal.pone.0354756",
  "fulltext_url": null,
  "id": "05pwfpn58sc0a",
  "issns": [
    "1932-6203"
  ],
  "landing_url": "https://doi.org/10.1371/journal.pone.0354756",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1311|Genetic and phenotypic traits in livestock"
    ],
    [
      "plos",
      "/Biology and life sciences/Agriculture/Animal products/Meat"
    ],
    [
      "plos",
      "/Biology and life sciences/Biochemistry/Lipids/Lipid metabolism"
    ],
    [
      "plos",
      "/Biology and life sciences/Biochemistry/Metabolism/Lipid metabolism"
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    [
      "plos",
      "/Biology and life sciences/Biochemistry/Metabolism/Metabolic pathways"
    ],
    [
      "plos",
      "/Biology and life sciences/Biochemistry/Proteins/Muscle proteins"
    ],
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    ],
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      "/Biology and life sciences/Computational biology/Genome analysis/Transcriptome analysis"
    ],
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      "plos",
      "/Biology and life sciences/Genetics/Genomics/Genome analysis/Transcriptome analysis"
    ],
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      "/Biology and life sciences/Molecular biology/Molecular biology techniques/Molecular biology assays and analysis techniques/Gene expression and vector techniques/Protein expression"
    ],
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      "plos",
      "/Biology and life sciences/Nutrition/Diet/Food/Meat"
    ],
    [
      "plos",
      "/Biology and life sciences/Organisms/Eukaryota/Animals/Vertebrates/Amniotes/Mammals/Swine"
    ],
    [
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      "/Biology and life sciences/Zoology/Animals/Vertebrates/Amniotes/Mammals/Swine"
    ],
    [
      "plos",
      "/Medicine and health sciences/Nutrition/Diet/Food/Meat"
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      "/Research and analysis methods/Database and informatics methods/Biological databases/Proteomic databases"
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      "plos",
      "/Research and analysis methods/Molecular biology techniques/Molecular biology assays and analysis techniques/Gene expression and vector techniques/Protein expression"
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  "pdf_url": "https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0354756&type=printable",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-18",
  "publisher": "Public Library of Science",
  "ris": "https://research.draconitic.com/paper/05pwfpn58sc0a.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:49Z",
      "native_id": "10.1371/journal.pone.0354756",
      "source": "plos",
      "url": "https://doi.org/10.1371/journal.pone.0354756"
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  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "plos:metabolism",
      "subfield": "enzymology-metabolism"
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    {
      "field": "biochemistry",
      "origin": "plos:proteomics",
      "subfield": "proteomics-metabolomics"
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    {
      "field": "medicine",
      "origin": "plos:nutrition",
      "subfield": "nutrition"
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  ],
  "title": "Integrated analysis of transcriptomic and proteomic profiles identifies divergent regulatory networks for intramuscular fat in Chinese versus Western Pigs",
  "title_text": "Integrated analysis of transcriptomic and proteomic profiles identifies divergent regulatory networks for intramuscular fat in Chinese versus Western Pigs",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:49Z",
  "url": "https://research.draconitic.com/paper/05pwfpn58sc0a",
  "venue": "PLOS One",
  "versions": []
}