{
  "abstract": "Despite substantial treatment improvements, the 5-year survival rate for metastatic osteosarcoma (OS) is still as low as 20%. Immunocompetent genetic mouse models of OS have been introduced, enabling in vivo OS studies. Here, we present the ROCCIA mouse, a quintuple-mutant extension of these models that develops multifluorescent OS tumors, allowing distinct fluorescently labeled OS cell populations to be identified and traced both within tumors and in the circulation. In addition, this model enables postnatal inactivation of the p53 and Rb1 genes, thus resembling development and progression of OS in humans. Using this model, we show that osterix ( Osx )–expressing fluorescent tumor cells appear in circulation upon OS development and predominate in lung metastases, thus representing a potential target for future therapies. We also show that the immunological landscape of this model’s tumor microenvironment mimics the previously described human OS microenvironment. Therefore, this immunocompetent, quintuple mutant, multifluorescent mouse model represents a powerful resource to analyze OS tumor development and metastasis in real time and to test translational therapies.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Oral and Craniofacial Sciences, University of Pittsburgh School of Dental Medicine, Pittsburgh, PA, USA.",
    "Department of Periodontics and Preventive Dentistry, University of Pittsburgh School of Dental Medicine, Pittsburgh, PA, USA.",
    "Center for Craniofacial Regeneration, University of Pittsburgh School of Dental Medicine, Pittsburgh, PA, USA.",
    "Center for Systems Biology and Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.",
    "Department of Immunology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.",
    "Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.",
    "Hillman Cancer Center, University of Pittsburgh Medical Center, Pittsburgh, PA, USA.",
    "Department of Orthopaedic Surgery, University of Pittsburgh Medical Center, Pittsburgh, PA, USA.",
    "Pittsburgh Center for Interdisciplinary Bone and Mineral Research, University of Pittsburgh, Pittsburgh, PA, USA.",
    "Department of Medicine, Division of Hematology and Oncology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA."
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 11,
  "authors": [
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Taiana C. Leite",
      "orcid": "0000-0001-5435-2317"
    },
    {
      "affs": [
        3
      ],
      "name": "Jue Hou",
      "orcid": "0000-0003-4417-7134"
    },
    {
      "affs": [
        1,
        2
      ],
      "name": "Xuehui Geng"
    },
    {
      "affs": [
        1,
        2
      ],
      "name": "Luigi Mancinelli",
      "orcid": "0000-0002-7264-4978"
    },
    {
      "affs": [
        4
      ],
      "name": "Abigail Sedlacek",
      "orcid": "0000-0001-5623-6723"
    },
    {
      "affs": [
        4
      ],
      "name": "Devanshi A. Nayak",
      "orcid": "0000-0002-7897-0758"
    },
    {
      "affs": [
        5
      ],
      "name": "Karen E. Schoedel",
      "orcid": "0000-0001-5398-8962"
    },
    {
      "affs": [
        6,
        7
      ],
      "name": "Kurt R. Weiss"
    },
    {
      "affs": [
        4
      ],
      "name": "Robert J. Binder",
      "orcid": "0000-0003-0857-3949"
    },
    {
      "affs": [
        3
      ],
      "name": "Charles P. Lin",
      "orcid": "0000-0002-7450-627X"
    },
    {
      "affs": [
        1,
        2,
        6,
        8,
        9
      ],
      "name": "Giuseppe Intini",
      "orcid": "0000-0002-6862-7040"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/06ntce49xmrdn.bib",
  "cited_by": 0,
  "doi": "10.1126/sciadv.aed6938",
  "fulltext_url": null,
  "id": "06ntce49xmrdn",
  "issns": [
    "2375-2548"
  ],
  "landing_url": "https://www.science.org/doi/10.1126/sciadv.aed6938",
  "language": null,
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2740|Sarcoma Diagnosis and Treatment"
    ]
  ],
  "openalex": "W7215772927",
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-02",
  "publisher": "American Association for the Advancement of Science (AAAS)",
  "ris": "https://research.draconitic.com/paper/06ntce49xmrdn.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:41Z",
      "native_id": "10.1126/sciadv.aed6938",
      "source": "crossref",
      "url": "https://www.science.org/doi/10.1126/sciadv.aed6938"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2740|Sarcoma Diagnosis and Treatment",
      "subfield": "general"
    }
  ],
  "title": "An immunocompetent, multifluorescent resource for real-time analysis of osteosarcoma progression, metastasis, and response to therapy",
  "title_text": "An immunocompetent, multifluorescent resource for real-time analysis of osteosarcoma progression, metastasis, and response to therapy",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:41Z",
  "url": "https://research.draconitic.com/paper/06ntce49xmrdn",
  "venue": "Science Advances",
  "versions": []
}