{
  "abstract": "The extent to which diabetic foot ulcer transcriptomic findings agree across cohorts remains uncertain. We compared an RNA sequencing cohort of 13 ulcer and eight diabetic foot skin samples with a microarray cohort of six ulcer and three diabetic foot skin samples. Single-cell data from 26 participants were analyzed after aggregating gene counts within each patient and cell type. A further cohort of 10 ulcer and 11 healthy foot-skin samples provided a descriptive directional comparison. Healing-status comparisons were exploratory. The primary analysis selected 4,704 differentially expressed gene identifiers; 2,943 were represented among the 17,502 gene symbols shared with the microarray cohort. Of these, 1,254 had concordant directions and nominal microarray P < 0.05, including 1,028 with microarray false discovery rate < 0.05. Direction agreement was 70.2%, and overall effect-rank correlation was modest (Spearman ρ = 0.29). Five Hallmark gene sets met the cross-cohort enrichment criteria. More specific functional analysis of the stricter supported set highlighted cornified-envelope formation, keratinization and inflammatory-cell migration. In the additional cohort, 611 of 823 represented genes from this set agreed in direction, although its comparator differed. Twelve keratinocyte genes met the cell-type differential-expression threshold; this finding does not establish cell-type exclusivity. Healing-status results were sensitive to covariate adjustment. These data support partial concordance in ulcer-associated epithelial, inflammatory and metabolic expression patterns. Limited cohort coverage, comparator differences and unresolved confounding preclude a universal disease signature, healing predictor or causal interpretation.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Endocrinology, Zhangjiajie People’s Hospital, Zhangjiajie, Hunan, China",
    "Department of Gastroenterology, Shenzhen Xinhua Hospital, Shenzhen, Guangdong, China"
  ],
  "article_type": "Research Article",
  "arxiv": null,
  "author_total": 2,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Shiqin Huang"
    },
    {
      "affs": [
        0
      ],
      "name": "Yukun Li",
      "orcid": "0009-0003-3089-2919"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/06r3sjtw7swb2.bib",
  "cited_by": 0,
  "doi": "10.1371/journal.pone.0356328",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13712624/",
  "id": "06r3sjtw7swb2",
  "issns": [
    "1932-6203"
  ],
  "landing_url": "https://doi.org/10.1371/journal.pone.0356328",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Anatomy"
    ],
    [
      "heading",
      "Animal Cells"
    ],
    [
      "heading",
      "Bioassays and Physiological Analysis"
    ],
    [
      "heading",
      "Biological Tissue"
    ],
    [
      "heading",
      "Biology and Life Sciences"
    ],
    [
      "heading",
      "Biology and life sciences"
    ],
    [
      "heading",
      "Cell Biology"
    ],
    [
      "heading",
      "Cellular Types"
    ],
    [
      "heading",
      "Clinical Medicine"
    ],
    [
      "heading",
      "Comparators"
    ],
    [
      "heading",
      "Diabetes Mellitus"
    ],
    [
      "heading",
      "Electronics"
    ],
    [
      "heading",
      "Electronics Engineering"
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    [
      "heading",
      "Endocrine Disorders"
    ],
    [
      "heading",
      "Endocrinology"
    ],
    [
      "heading",
      "Engineering and Technology"
    ],
    [
      "heading",
      "Epithelial Cells"
    ],
    [
      "heading",
      "Epithelium"
    ],
    [
      "heading",
      "Keratinocytes"
    ],
    [
      "heading",
      "Medical Conditions"
    ],
    [
      "heading",
      "Medicine and Health Sciences"
    ],
    [
      "heading",
      "Metabolic Disorders"
    ],
    [
      "heading",
      "Microarrays"
    ],
    [
      "heading",
      "Molecular biology"
    ],
    [
      "heading",
      "Molecular biology assays and analysis techniques"
    ],
    [
      "heading",
      "Molecular biology techniques"
    ],
    [
      "heading",
      "Nucleic acid analysis"
    ],
    [
      "heading",
      "Physiological Processes"
    ],
    [
      "heading",
      "Physiology"
    ],
    [
      "heading",
      "RNA analysis"
    ],
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      "heading",
      "RNA sequencing"
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    [
      "heading",
      "Research and Analysis Methods"
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      "heading",
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      "heading",
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    ],
    [
      "heading",
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    [
      "heading",
      "Tissue Repair"
    ],
    [
      "heading",
      "Ulcers"
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    [
      "heading",
      "Wound Healing"
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    [
      "openalex",
      "2712|Diabetic Foot Ulcer Assessment and Management"
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    [
      "plos",
      "/Biology and life sciences/Anatomy/Biological tissue/Epithelium/Epithelial cells/Keratinocytes"
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      "/Biology and life sciences/Cell biology/Cellular types/Animal cells/Epithelial cells/Keratinocytes"
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      "/Biology and life sciences/Molecular biology/Molecular biology techniques/Molecular biology assays and analysis techniques/Nucleic acid analysis/RNA analysis"
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      "/Medicine and health sciences/Clinical medicine/Signs and symptoms/Ulcers"
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      "/Medicine and health sciences/Endocrinology/Endocrine disorders/Diabetes mellitus"
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      "/Medicine and health sciences/Medical conditions/Metabolic disorders/Diabetes mellitus"
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      "/Research and analysis methods/Bioassays and physiological analysis/Microarrays"
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      "/Research and analysis methods/Molecular biology techniques/Molecular biology assays and analysis techniques/Nucleic acid analysis/RNA analysis"
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  "pdf_url": "https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0356328&type=printable",
  "pmcid": "PMC13712624",
  "pmid": "42945178",
  "published": "2026-10-27",
  "publisher": "Public Library of Science",
  "ris": "https://research.draconitic.com/paper/06r3sjtw7swb2.ris",
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  "subjects": [
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  "title": "Cross-platform comparison of diabetic foot ulcer transcriptomes identifies partially concordant disease-associated signals",
  "title_text": "Cross-platform comparison of diabetic foot ulcer transcriptomes identifies partially concordant disease-associated signals",
  "type": "peer-reviewed",
  "updated_at": "2026-10-31T12:23:10Z",
  "url": "https://research.draconitic.com/paper/06r3sjtw7swb2",
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