{
  "abstract": "Cells experience external forces that deform the plasma membrane to which they adapt by reorganizing their actin cytoskeleton. Here, using the extracellular bacterium Neisseria meningitidis as a model system, we explore how this bacterium reorganizes the cortical actin cytoskeleton subsequently to mechanical membrane deformations. Meningococci trigger the formation of tubular cellular plasma membrane protrusions by a previously described adhesion-driven process known as one-dimensional wetting. Cryo-electron tomography reveals that in epithelial cells such a deformation of the plasma membrane leads to the formation of F-actin bundles. In contrast, in endothelial cells a branched F-actin network is formed. By combining high resolution photonic microscopy approaches with genetic and drug perturbations in endothelial cells, we demonstrate that Arp2/3 activity is necessary for forming this branched network. We demonstrate the role of the nucleating-promoting factor N-WASP downstream of Cdc42. Proteomic analyses reveal the contribution of the small GTPase Arf1. Taken together, our results delineate an Arf1-Cdc42-N-WASP-Arp2/3 pathway that links mechanical plasma membrane deformation to the subsequent reorganization of a cortical branched F-actin network in endothelial cells.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 19,
  "authors": [
    {
      "name": "Margot Sahnine"
    },
    {
      "name": "Morgane Crochet"
    },
    {
      "name": "Stéphane Tachon"
    },
    {
      "name": "Sylvie Goussard"
    },
    {
      "name": "Esthel Pénard"
    },
    {
      "name": "Cyril Scandola"
    },
    {
      "name": "Magalie Duchateau"
    },
    {
      "name": "Quentin Giai Gianetto"
    },
    {
      "name": "Audrey Salles"
    },
    {
      "name": "Gaël Moneron"
    },
    {
      "name": "Léa Swistak"
    },
    {
      "name": "Arthur Charles-Orszag"
    },
    {
      "name": "Adeline Mallet"
    },
    {
      "name": "Mariette Matondo"
    },
    {
      "name": "Jean-Yves Tinevez"
    },
    {
      "name": "Daria Bonazzi"
    },
    {
      "name": "Anna Sartori-Rupp"
    },
    {
      "name": "Guillaume Duménil"
    },
    {
      "name": "Dorian Obino"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/078w8akpw2zmb.bib",
  "cited_by": 0,
  "doi": "10.1038/s44319-026-00843-z",
  "fulltext_url": null,
  "id": "078w8akpw2zmb",
  "issns": [
    "1469-221X",
    "1469-3178"
  ],
  "landing_url": "https://hal.science/hal-05785011v1",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "hal",
      "0.sdv"
    ],
    [
      "hal",
      "1.sdv.bc"
    ],
    [
      "hal",
      "2.sdv.bc.bc"
    ],
    [
      "openalex",
      "1307|Cellular Mechanics and Interactions"
    ]
  ],
  "openalex": "W7165863193",
  "pdf_url": "https://hal.science/hal-05785011/document",
  "pmcid": null,
  "pmid": "42350658",
  "published": "2026-06-25",
  "publisher": "EMBO Press",
  "ris": "https://research.draconitic.com/paper/078w8akpw2zmb.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:50Z",
      "native_id": "hal-05785011",
      "source": "hal",
      "url": "https://hal.science/hal-05785011v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "hal:1.sdv.bc",
      "subfield": "cell-biology"
    }
  ],
  "title": "Arf1 is involved in Neisseria meningitidis-induced cortical branched F-actin network reorganization",
  "title_text": "Arf1 is involved in Neisseria meningitidis-induced cortical branched F-actin network reorganization",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:50Z",
  "url": "https://research.draconitic.com/paper/078w8akpw2zmb",
  "venue": "EMBO Reports",
  "versions": []
}