{
  "abstract": "Background\n\nThere are limited data on the molecular epidemiology of New Delhi metallo-β-lactamase (NDM)-producing carbapenem-resistant Enterobacterales (CRE) in Latin America, despite the high burden of NDM-CRE in the region. This study characterized bla NDM-harboring plasmids from CRE from patients and the environment of an Argentine hospital.Method\n\nWe performed whole-genome sequencing of 40 environmental isolates (Klebsiella complex, n = 11; Enterobacter cloacae, complex n = 16; Citrobacter freundii, n = 10; Serratia marcescens, n = 3) and 4 clinical isolates (Klebsiella pneumoniae, n = 4) recovered from the environment and rectal surveillance samples of patients in the same intensive care units (ICUs) at a tertiary care center in Argentina, July 2023-February 2024, respectively. Representative genomic groups underwent Oxford Nanopore sequencing and hybrid genome assembly for complete plasmid characterization. Resolved bla NDM-harboring plasmids were compared across species and publicly available reference sequences to characterize plasmid relatedness and dissemination patterns.Results\n\nTen representative genomic groups were identified across species, including 7 defined sequence types and 3 assigned in this study. bla NDM-1 and bla NDM-5 were present in 72.5% (29/40) and 27.5% (11/40) of environmental isolates, respectively (all 4 clinical isolates were bla NDM-5). Hybrid assembly of the representative genomic groups revealed bla NDM-1 was located on highly conserved plasmids belonging to the IncC incompatibility group across multiple CRE species. bla NDM-5 was restricted to Klebsiella spp. and was associated with structurally diverse plasmids including IncF- and IncHI1B/IncFIB-type backbones in both environmental and patient isolates.Conclusions\n\nThese findings highlight the ICU environment as a reservoir for bla NDM plasmids.",
  "abstract_shown": true,
  "affiliations": [
    "Servicio Antimicrobianos, National Reference Laboratory in Antimicrobial Resistance (NRLAR), National Institute of Infectious Diseases (INEI), ANLIS \"Dr. Carlos G. Malbrán\", Buenos Aires, Argentina.",
    "Department of Medicine, Division of Infectious Diseases, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.",
    "UO Centro Nacional de Genómica y Bioinformática, National Center of Genomics and Bioinformatics, ANLIS \"Dr. Carlos G. Malbrán\", Buenos Aires, Argentina.",
    "Microbiología, Servicio Laboratorio, Hospital Británico de Buenos Aires, Buenos Aires, Argentina.",
    "Division of Healthcare Quality Promotion, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 10,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Florencia Martino",
      "orcid": "0009-0006-1381-6326"
    },
    {
      "affs": [
        2
      ],
      "name": "Denise De Belder",
      "orcid": "0000-0002-5568-4804"
    },
    {
      "affs": [
        3
      ],
      "name": "Renata Monge"
    },
    {
      "affs": [
        3
      ],
      "name": "Agustina Forastiero",
      "orcid": "0009-0003-2722-1751"
    },
    {
      "affs": [
        4
      ],
      "name": "Molly Patrick",
      "orcid": "0000-0002-9623-5909"
    },
    {
      "affs": [
        4
      ],
      "name": "Kara A Moser",
      "orcid": "0000-0002-9247-7388"
    },
    {
      "affs": [
        0
      ],
      "name": "Alejandra Corso",
      "orcid": "0000-0003-1183-6746"
    },
    {
      "affs": [
        0
      ],
      "name": "Fernando Pasteran",
      "orcid": "0000-0001-5840-5869"
    },
    {
      "affs": [
        1
      ],
      "name": "Matthew Robinson",
      "orcid": "0000-0003-0376-8560"
    },
    {
      "affs": [
        1
      ],
      "name": "Valeria Fabre",
      "orcid": "0000-0001-7374-9678"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/099jvxx31r3a3.bib",
  "cited_by": 0,
  "doi": "10.1093/ofid/ofag663",
  "fulltext_url": "https://europepmc.org/articles/PMC13678220",
  "id": "099jvxx31r3a3",
  "issns": [
    "2328-8957"
  ],
  "landing_url": "https://europepmc.org/article/MED/42898562",
  "language": "eng",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1313|Antibiotic Resistance in Bacteria"
    ]
  ],
  "openalex": "W7220987262",
  "pdf_url": "https://europepmc.org/articles/PMC13678220?pdf=render",
  "pmcid": "PMC13678220",
  "pmid": "42898562",
  "published": "2026-10-07",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/099jvxx31r3a3.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:37Z",
      "native_id": "MED:42898562",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42898562"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:1313|Antibiotic Resistance in Bacteria",
      "subfield": "general"
    }
  ],
  "title": "New Delhi Metallo-β-Lactamase Plasmidomes of Intensive Care Unit Clinical and Environmental Enterobacterales Isolates.",
  "title_text": "New Delhi Metallo-β-Lactamase Plasmidomes of Intensive Care Unit Clinical and Environmental Enterobacterales Isolates.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:37Z",
  "url": "https://research.draconitic.com/paper/099jvxx31r3a3",
  "venue": "Open forum infectious diseases",
  "versions": []
}