{
  "abstract": "Electrochemical aptamer-based (E-AB) biosensors and clustered regularly interspaced short palindromic repeats (CRISPR) systems have independently advanced molecular diagnostics; however, their integration is less explored. Here, we present a CRISPR-enabled electrochemical sensing platform that directly links CRISPR Cas12a-mediated cleavage of non-specific aptamers to a change of charge transfer at the electrode interface. Methylene blue (MB)-labeled aptamers are immobilized on a gold electrode, forming an electrochemically surface-confined system. Upon target-activated CRISPR cleavage, MB-labeled aptamers are released into the medium, transforming the system into a diffusion-controlled regime. This transition is directly captured through real-time electrochemical monitoring, where alternating current voltammetry reveals a progressive decrease in surface-bound redox reporters, while cyclic voltammetry simultaneously shows an increase in faradaic current due to the accumulation of freely diffusing MB species. Scan rate-dependent analysis confirms a shift from non-ideal surface-limited behavior to diffusion-controlled kinetics, consistent with the Randles–Ševčík relationship. We propose an adsorption-coupled transport model, which supports this interpretation by showing that the plausible post-cleavage voltammetric response is best described by near-surface confinement of released MB-tagged fragments rather than by pure bulk diffusion alone. Dose-response analysis shows that cyclic voltammetry achieves a improved limit of detection (LOD) of approximately , outperforming square wave voltammetry ( across a broader – range), underscoring CV as the more sensitive readout for quantitative biosensing with this platform. By leveraging the exceptional molecular specificity of CRISPR alongside the high sensitivity of E-AB sensors, this work establishes a new sensing paradigm in which conventional steady-state voltammetry can be used for analytical detection.",
  "abstract_shown": true,
  "affiliations": [
    "Materials Research Institute",
    "The Pennsylvania State University",
    "Department of Electrical Engineering",
    "Department of Biomedical Engineering"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 2,
  "authors": [
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Pouya Soltan Khamsi",
      "orcid": "0000-0002-6121-338X"
    },
    {
      "affs": [
        0,
        1,
        2,
        3
      ],
      "name": "Aida Ebrahimi"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/09azhkmh61npp.bib",
  "cited_by": 0,
  "doi": "10.26434/chemrxiv.15009466/v1",
  "fulltext_url": null,
  "id": "09azhkmh61npp",
  "issns": [],
  "landing_url": "https://chemrxiv.org/doi/full/10.26434/chemrxiv.15009466/v1",
  "language": null,
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Advanced biosensing and bioanalysis techniques"
    ]
  ],
  "openalex": "W7214322805",
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-24",
  "publisher": "American Chemical Society (ACS)",
  "ris": "https://research.draconitic.com/paper/09azhkmh61npp.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:21Z",
      "native_id": "10.26434/chemrxiv.15009466",
      "source": "chemrxiv",
      "url": "https://chemrxiv.org/doi/full/10.26434/chemrxiv.15009466/v1"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Advanced biosensing and bioanalysis techniques",
      "subfield": "molecular-biology"
    }
  ],
  "title": "CRISPR-Enabled Transition to Adsorption-Coupled Redox Transport: Toward the Use of Conventional Voltammetry in Electrochemical Biosensors",
  "title_text": "CRISPR-Enabled Transition to Adsorption-Coupled Redox Transport: Toward the Use of Conventional Voltammetry in Electrochemical Biosensors",
  "type": "preprint",
  "updated_at": "2026-10-30T12:17:21Z",
  "url": "https://research.draconitic.com/paper/09azhkmh61npp",
  "venue": "ChemRxiv",
  "versions": []
}