{
  "abstract": "Host factors determine pathogen tropism but knowledge of host genes facilitating enteropathogenic Escherichia coli (EPEC) infection is limited. To identify host determinants required for infection by EPEC, we performed two independent genome-scale CRISPR-Cas9 screens. Both converged on host N-glycosylation as a critical requirement for infection. Genetic or pharmacological inhibition of N-glycan maturation revealed that N-glycosylation promotes infection through two distinct pathways. First, N-linked poly-N-acetyllactosamine glycans serve as receptors for the major EPEC adhesin, the bundle-forming pilus (BFP). Second, perturbation of N-glycosylation compromises host membrane structure, resulting in reduced type III secretion system activity, independently of host cell attachment. Using epithelial monolayers derived from human colon and jejunum organoids, we further show that EPEC attachment inversely correlates with terminal mannose exposure, suggesting glycan composition may be involved in determining intestinal tropism. Together, these findings establish host N-glycan maturation as a key host pathway exploited during enteric infection.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Microbiology and Molecular Genetics, Institute for Medical Research Israel-Canada, Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem 9112102, Israel",
    "Hadassah Organoid Center, The Hadassah Medical Organization, Jerusalem 9166100, Israel",
    "Department of Pediatric Gastroenterology, Hadassah Medical Organization and Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem 9166100, Israel",
    "Department of Developmental Biology and Cancer Research, Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem 9166100, Israel",
    "Department of Microbiology, Harvard Medical School, Boston, MA, USA",
    "Division of Infectious Diseases, Brigham & Women’s Hospital, Boston, MA, USA",
    "Department for Medical Biotechnology, The Technical University Berlin, Gustav-Meyer-Allee 25, 13355 Berlin, Germany",
    "Howard Hughes Medical Institute, Boston, MA, USA"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 14,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Klil Cohen Yaron"
    },
    {
      "affs": [
        0
      ],
      "name": "Yaakov Socol"
    },
    {
      "affs": [
        0
      ],
      "name": "Noam Yedidi"
    },
    {
      "affs": [
        0
      ],
      "name": "Michal Bejerano-Sagie"
    },
    {
      "affs": [
        0
      ],
      "name": "Miriam Ravins"
    },
    {
      "affs": [
        1
      ],
      "name": "Shira Fine-Leebhoff"
    },
    {
      "affs": [
        1,
        2
      ],
      "name": "Liron Birimberg-Schwartz"
    },
    {
      "affs": [
        1,
        3
      ],
      "name": "Myriam Grunewald"
    },
    {
      "affs": [
        4,
        5
      ],
      "name": "Karthik Hullahalli"
    },
    {
      "affs": [
        6
      ],
      "name": "Afonso de Sousa Vieira"
    },
    {
      "affs": [
        4,
        5,
        7
      ],
      "name": "Matthew K. Waldor"
    },
    {
      "affs": [
        6
      ],
      "name": "Sina Bartfeld"
    },
    {
      "affs": [
        0
      ],
      "name": "Sigal Ben-Yehuda"
    },
    {
      "affs": [
        0
      ],
      "name": "Ilan Rosenshine"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/09r571pfvbbs5.bib",
  "cited_by": 0,
  "doi": "10.1016/j.celrep.2026.118043",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13714174/",
  "id": "09r571pfvbbs5",
  "issns": [
    "2211-1247"
  ],
  "landing_url": "https://doi.org/10.1016/j.celrep.2026.118043",
  "language": "en",
  "licence": "CC-BY-NC-4.0",
  "licence_label": "CC BY-NC 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Article"
    ],
    [
      "openalex",
      "1310|Escherichia coli research studies"
    ]
  ],
  "openalex": "W7214777257",
  "pdf_url": null,
  "pmcid": "PMC13714174",
  "pmid": "42814570",
  "published": "2026-09-29",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/09r571pfvbbs5.ris",
  "sources": [
    {
      "first_seen": "2026-11-01T12:21:43Z",
      "native_id": "PMC13714174",
      "source": "pmc",
      "url": "https://doi.org/10.1016/j.celrep.2026.118043"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:1310|Escherichia coli research studies",
      "subfield": "endocrinology-metabolism"
    }
  ],
  "title": "Dual roles of host N-glycosylation in enteropathogenic Escherichia coli infection",
  "title_text": "Dual roles of host N-glycosylation in enteropathogenic Escherichia coli infection",
  "type": "peer-reviewed",
  "updated_at": "2026-11-01T12:21:43Z",
  "url": "https://research.draconitic.com/paper/09r571pfvbbs5",
  "venue": "Cell Reports",
  "versions": []
}