{
  "abstract": "MicroRNAs (miRNAs) are promising biomarkers for cancer diagnosis, and their sensitive and accurate detection is important for early cancer diagnosis. However, achieving sensitive miRNA detection and logic analysis while maintaining a simple workflow remains challenging. In this study, catalytic hairpin assembly (CHA) and hybridization chain reaction (HCR) were sequentially coupled to construct a dual-stage enzyme-free amplification circuit and integrated with a label-free fluorescence reporting strategy based on graphene oxide and SYBR Green I for logical recognition and sensitive detection of miR-21. CHA provides specific target recognition and primary signal amplification, and its product triggers the downstream HCR to generate dsDNA nanowires. The resulting dsDNA provides abundant SYBR Green I binding sites and enables continuous signal enhancement. Molecular simulations further supported the feasibility and kinetic behavior of the sequential cascade pathway. The modular cascade also produced a three-input AND logic response, demonstrating controllable logic operation. Under isothermal, enzyme-free, and label-free conditions, the sensor achieved a limit of detection of 184 pM and distinguished highly similar miRNA sequences at the single-base level. This CHA-HCR system provides a simple and reliable approach to miRNA analysis and shows potential for the convenient, sensitive detection of trace biomarkers.",
  "abstract_shown": true,
  "affiliations": [
    "College of Life Science, Shaanxi Normal University Xi'an Shaanxi 710119 PR China dongyf@snnu.edu.cn.",
    "College of Electrical and Information Engineering, Zhengzhou University of Light Industry Zhengzhou Henan 450000 PR China."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Can Qiao",
      "orcid": "0009-0005-8801-8196"
    },
    {
      "affs": [
        0
      ],
      "name": "Zhenliang Zhang"
    },
    {
      "affs": [
        0
      ],
      "name": "Yidan Sang"
    },
    {
      "affs": [
        0
      ],
      "name": "Mengyang Hu",
      "orcid": "0000-0002-9804-150X"
    },
    {
      "affs": [
        0
      ],
      "name": "Yafei Dong"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Luhui Wang"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/09xbdcr7k7bbn.bib",
  "cited_by": 0,
  "doi": "10.1039/d6ra08323d",
  "fulltext_url": "https://europepmc.org/articles/PMC13678498",
  "id": "09xbdcr7k7bbn",
  "issns": [
    "2046-2069"
  ],
  "landing_url": "https://europepmc.org/article/MED/42898965",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Chemistry"
    ],
    [
      "openalex",
      "1312|Advanced biosensing and bioanalysis techniques"
    ]
  ],
  "openalex": "W7222934094",
  "pdf_url": "https://europepmc.org/articles/PMC13678498?pdf=render",
  "pmcid": "PMC13678498",
  "pmid": "42898965",
  "published": "2026-10-19",
  "publisher": "Royal Society of Chemistry",
  "ris": "https://research.draconitic.com/paper/09xbdcr7k7bbn.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:33Z",
      "native_id": "MED:42898965",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42898965"
    },
    {
      "first_seen": "2026-11-07T12:23:33Z",
      "native_id": "PMC13678498",
      "source": "pmc",
      "url": "https://doi.org/10.1039/d6ra08323d"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Advanced biosensing and bioanalysis techniques",
      "subfield": "molecular-biology"
    }
  ],
  "title": "A label-free fluorescent sensor for miR-21 based on sequentially coupled CHA-HCR dual-stage amplification and synergistic signal regulation by graphene oxide and SYBR Green I.",
  "title_text": "A label-free fluorescent sensor for miR-21 based on sequentially coupled CHA-HCR dual-stage amplification and synergistic signal regulation by graphene oxide and SYBR Green I.",
  "type": "peer-reviewed",
  "updated_at": "2026-11-08T12:21:01Z",
  "url": "https://research.draconitic.com/paper/09xbdcr7k7bbn",
  "venue": "RSC advances",
  "versions": []
}