{
  "abstract": "Background: Mesenchymal stromal cell-derived exosomes (MSC-EXOs), also called Extracellular Vesicles (EVs), exhibit anti-inflammatory effects in various diseases and are being developed for clinical use. For instance, MSC-EXO promotes skin healing post-laser therapy and improves outcomes in severe COVID-19. Selecting an optimal cellular source is critical for the therapeutic development of MSC-EXO. It has been suggested that GMP-produced MSC-exosomes have slightly different molecular content. This study therefore aimed to compare adipose tissue-derived MSC-EXO (ASC-EXO) from three healthy donors, isolated and characterized under GMP conditions.\n\nMethods: Exosomes were analyzed by nano-tracking analysis (NTA), cryo-electron microscopy, tetraspanin profiling, and multi-omics (proteomics, lipidomics, small RNA sequencing), as well as bioinformatics. Functional assays quantified anti-inflammatory effects in RAW264.7 macrophages and collagen production by human dermal fibroblasts (HDF). In vivo efficacy of ASC-EXOs was evaluated in a house dust mite antigen-induced atopic dermatitis mouse model through histopathological evaluation of ear thickness and differential cell counting.\n\nResults: No significant batch differences were observed in ASC-EXO yield or characteristics. Omics analyses revealed minor variations in protein, lipid, and small RNA cargo among the three batches, but GO-term bioinformatics indicated highly similar functional profiles. IL-6 suppression in RAW264.7 cells and cell proliferation and collagen production by HDF were similar among the ASC-EXO batches. CD73 enzymatic activity was consistent among batches. In vivo, the ASC-EXOs reduced skin thickness and eosinophilia in an atopic dermatitis model, with similar effects among the batches.\n\nConclusions: In summary, ASC-EXOs from all batches demonstrated comparable anti-inflammatory and collagen-modulating effects in vitro, and similar inhibition of atopic dermatitis signs in vivo. We suggest that biological efficacy combined with bioinformatics analysis should guide MSC-EXO therapeutic quality control assays. These findings support the development of ASC-EXOs for clinical applications.\n\nSupplementary Information: The online version contains supplementary material available at https://doi.org/10.1186/s13287-026-05104-3.",
  "abstract_shown": true,
  "affiliations": [
    "ExoCoBio Exosome Institute (EEI), ExoCoBio Inc, STE 306, 19 Gasan Digital 1-Ro, Geumcheon-Gu, Seoul, 08594 Republic of Korea",
    "Department of Food Science and Nutrition, Convergence Program of Material Science for Medicine and Pharmaceutics, Hallym University, Chuncheon, 24252 Republic of Korea",
    "Krefting Research Centre, The Sahlgrenska Academy, University of Gothenburg, Box 424, 405 30 Gothenburg, Sweden"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 11,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Byong Seung Cho"
    },
    {
      "affs": [
        0
      ],
      "name": "Jun Ho Lee"
    },
    {
      "affs": [
        1
      ],
      "name": "Kyong-Oh Shin"
    },
    {
      "affs": [
        1
      ],
      "name": "Kyungho Park"
    },
    {
      "affs": [
        0
      ],
      "name": "Kyojin Lee"
    },
    {
      "affs": [
        0
      ],
      "name": "Ella Shin"
    },
    {
      "affs": [
        0
      ],
      "name": "Hyunju Lee"
    },
    {
      "affs": [
        0
      ],
      "name": "Healim Lee"
    },
    {
      "affs": [
        2
      ],
      "name": "Kyong-Su Park"
    },
    {
      "affs": [
        0
      ],
      "name": "Dae Hyun Ha"
    },
    {
      "affs": [
        2
      ],
      "name": "Jan Lötvall"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/09xj9r8xzwxam.bib",
  "cited_by": 0,
  "doi": "10.1186/s13287-026-05104-3",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13700134/",
  "id": "09xj9r8xzwxam",
  "issns": [
    "1757-6512"
  ],
  "landing_url": "https://doi.org/10.1186/s13287-026-05104-3",
  "language": "en",
  "licence": "CC-BY-NC-ND-4.0",
  "licence_label": "CC BY-NC-ND 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Research"
    ],
    [
      "openalex",
      "1312|Extracellular vesicles in disease"
    ]
  ],
  "openalex": "W7169838048",
  "pdf_url": null,
  "pmcid": "PMC13700134",
  "pmid": "42482105",
  "published": "2026-07-21",
  "publisher": "BMC",
  "ris": "https://research.draconitic.com/paper/09xj9r8xzwxam.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:20:38Z",
      "native_id": "PMC13700134",
      "source": "pmc",
      "url": "https://doi.org/10.1186/s13287-026-05104-3"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Extracellular vesicles in disease",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Consistent functional properties of MSC-exosomes from different batches revealed by comparative multi-omics, bioinformatics and functional tests",
  "title_text": "Consistent functional properties of MSC-exosomes from different batches revealed by comparative multi-omics, bioinformatics and functional tests",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:36:36Z",
  "url": "https://research.draconitic.com/paper/09xj9r8xzwxam",
  "venue": "Stem Cell Research & Therapy",
  "versions": []
}