{
  "abstract": "Neural organoids represent a promising approach for modelling neural circuits and neurological disease, yet their utility depends on whether they can recapitulate the molecular complexity of mature brain synapses. Here, we optimized a protocol to generate mature forebrain organoids from mouse embryonic stem cells and used time-resolved proteomics to define the emergence of synaptic proteins during maturation. Using fluorescence-activated synaptosome sorting coupled with mass spectrometry, we quantified the synapse-enriched proteome and found a high correlation with matched in vivo synaptic proteomes. These findings demonstrate that complex mature synaptic molecular architecture is present within an in vitro organoid environment.",
  "abstract_shown": true,
  "affiliations": [
    "MPI for Brain Research"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "name": "S. L. Giandomenico"
    },
    {
      "name": "M. van Oostrum"
    },
    {
      "name": "Q. Waselenchuk"
    },
    {
      "name": "I. Dalla Costa"
    },
    {
      "name": "J. D. Langer"
    },
    {
      "affs": [
        0
      ],
      "name": "E. M. Schuman"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/0agjzaj39cdxr.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.09.24.753785",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.09.24.753785v1.full",
  "id": "0agjzaj39cdxr",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.09.24.753785v1",
  "language": null,
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "neuroscience"
    ],
    [
      "openalex",
      "2804|Neuroscience and Neuropharmacology Research"
    ]
  ],
  "openalex": "W7214433814",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.09.24.753785v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-24",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/0agjzaj39cdxr.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:24:39Z",
      "native_id": "10.64898/2026.09.24.753785",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.09.24.753785v2"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:neuroscience",
      "subfield": "neuroscience"
    }
  ],
  "title": "Mouse Neural Organoids Model the Mature In Vivo Synaptic Proteome",
  "title_text": "Mouse Neural Organoids Model the Mature In Vivo Synaptic Proteome",
  "type": "preprint",
  "updated_at": "2026-10-30T12:29:06Z",
  "url": "https://research.draconitic.com/paper/0agjzaj39cdxr",
  "venue": "bioRxiv",
  "versions": []
}