{
  "abstract": "Molecular diagnosis of Lassa fever is complicated by extensive Lassa virus genetic diversity and limited laboratory capacity in endemic settings. The RealStar(C) Lassa Virus RT-PCR Kit 2.0 is a commonly used commercial dual-target assay for LASV detection, but its two-target format requires separate amplification reactions, increasing reagent use and workflow complexity. We present the diagnostic evaluation of the single-reaction, adaptive PANDAA Lassa Virus RT-PCR assay with the dual-target RealStar(C) Lassa Virus RT-PCR Kit 2.0 using 94 archived serum samples from Sierra Leone. 79 of these samples had valid paired results, and we report a positive percent agreement of 78.12% (95% CI 61.25%-88.98%), negative percent agreement of 93.62% (95% CI 82.84%-97.81%), and an overall agreement of 87.34% (95% CI 78.24%-92.98%); Cohen k; was 0.73 (95% CI 0.58-0.89). PANDAA showed substantial agreement and offers a potentially efficient single-well format, but further optimisation and prospective evaluation are needed.",
  "abstract_shown": true,
  "affiliations": [
    "Lassa Fever Unit, Kenema Government Hospital, Ministry of Health"
  ],
  "article_type": "PUBLISHAHEADOFPRINT",
  "arxiv": null,
  "author_total": 10,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "D. S. Grant"
    },
    {
      "name": "C. R. Thompson"
    },
    {
      "name": "J. Sandi"
    },
    {
      "name": "M. Manjia"
    },
    {
      "name": "T. Kallon"
    },
    {
      "name": "M. S. Kamara"
    },
    {
      "name": "I. Fofanah"
    },
    {
      "name": "T. R. Edwards"
    },
    {
      "name": "T. E. Fletcher"
    },
    {
      "name": "R. L. Byrne"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/0ax1b7egh7yk8.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.10.30.26366846",
  "fulltext_url": "https://www.medrxiv.org/content/10.64898/2026.10.30.26366846v1.full",
  "id": "0ax1b7egh7yk8",
  "issns": [],
  "landing_url": "https://www.medrxiv.org/content/10.64898/2026.10.30.26366846v1",
  "language": null,
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "medrxiv",
      "infectious diseases"
    ],
    [
      "openalex",
      "2725|Viral Infections and Vectors"
    ]
  ],
  "openalex": "W7225047300",
  "pdf_url": "https://www.medrxiv.org/content/10.64898/2026.10.30.26366846v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-30",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/0ax1b7egh7yk8.ris",
  "sources": [
    {
      "first_seen": "2026-11-02T12:20:44Z",
      "native_id": "10.64898/2026.10.30.26366846",
      "source": "medrxiv",
      "url": "https://www.medrxiv.org/content/10.64898/2026.10.30.26366846v2"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "medrxiv:infectious diseases",
      "subfield": "infectious-disease"
    }
  ],
  "title": "Clinical Evaluation of the PANDAA Lassa Virus RT-PCR Assay in Sierra Leone",
  "title_text": "Clinical Evaluation of the PANDAA Lassa Virus RT-PCR Assay in Sierra Leone",
  "type": "preprint",
  "updated_at": "2026-11-08T00:20:57Z",
  "url": "https://research.draconitic.com/paper/0ax1b7egh7yk8",
  "venue": "medRxiv",
  "versions": []
}