{
  "abstract": "Timely management of sepsis relies on rapid diagnosis of bloodstream infections (BSIs), including early pathogen identification and antimicrobial susceptibility testing (AST). The Vitek® REVEALTM is an innovative rapid AST platform that detects volatile organic compounds emitted by Gram-negative bacilli (GNB) directly from positive blood cultures. This study assessed the analytical performance and clinical contribution of the BioFire® BCID2 molecular panel and the Vitek® REVEALTM system in the diagnosis of GNB-BSI among intensive care unit (ICU) patients. We analyzed the prospective phase of the BacteREVEAL study, conducted between March and October 2023 at Nîmes University Hospital. Results from BioFire® BCID2 and Vitek® REVEALTM were compared to standard-of-care methods, including MALDI-TOF identification and direct disk diffusion AST. Performance metrics for Vitek® REVEALTM were evaluated according to ISO 20776–2:2007 standards. Among 94 ICU patients with a first episode of GNB-BSI, 50 monomicrobial cases were included. Escherichia coli was the most frequently identified species (n = 30, 60%), followed by Pseudomonas aeruginosa (n = 7, 14%). BioFire® BCID2 provided accurate identification for all isolates. The innovative tools reduced median turnaround times for identification by 19.0 h and for AST by 14.9 h. The mean time to result for Vitek® REVEALTM was 5.7 h. Overall categorical agreement with standard AST was 97.7%, with 8 very major errors, 8 major errors, and 2 minor errors. These findings support the integration of BioFire® BCID2 and Vitek® REVEALTM into routine workflows to accelerate GNB-BSI diagnosis and improve sepsis management.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Microbiology and Hospital Hygiene, Nîmes University Hospital, Nîmes, France",
    "Division of Anesthesia and Critical Care, UR-UM103 IMAGINE, University of Montpellier, Pain and Emergency Medicine, Nîmes University Hospital, Nîmes, France",
    "Department of Biostatistics, Epidemiology, Public Health, and Innovation in Methodology (BESPIM), Nîmes University Hospital, Nîmes, France",
    "VBIC, INSERM U1047, University of Montpellier, Nîmes, France"
  ],
  "article_type": "Research Article",
  "arxiv": null,
  "author_total": 10,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Massinissa Benyahia"
    },
    {
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        1
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      "name": "Claire Roger"
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    {
      "affs": [
        2
      ],
      "name": "Adeline Boutet-Dubois"
    },
    {
      "affs": [
        3
      ],
      "name": "Florian Salipante"
    },
    {
      "name": "Louis Oudiane"
    },
    {
      "name": "Matthieu Curtil Dit Galin"
    },
    {
      "name": "Rim Nsiri"
    },
    {
      "name": "Léo Brochon"
    },
    {
      "name": "Jean-Philippe Lavigne"
    },
    {
      "name": "Alix Pantel"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/0bem2gysfp91w.bib",
  "cited_by": 0,
  "doi": "10.1371/journal.pone.0356199",
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  "id": "0bem2gysfp91w",
  "issns": [
    "1932-6203"
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  "landing_url": "https://doi.org/10.1371/journal.pone.0356199",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1308|Bacterial Identification and Susceptibility Testing"
    ],
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      "plos",
      "/Biology and life sciences/Anatomy/Body fluids/Blood"
    ],
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      "plos",
      "/Biology and life sciences/Microbiology/Bacteriology/Gram negative bacteria"
    ],
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      "plos",
      "/Biology and life sciences/Microbiology/Medical microbiology/Microbial pathogens/Bacterial pathogens/Escherichia coli"
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      "plos",
      "/Biology and life sciences/Microbiology/Medical microbiology/Microbial pathogens/Bacterial pathogens/Pseudomonas aeruginosa"
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    ],
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      "plos",
      "/Biology and life sciences/Microbiology/Microbial control/Antimicrobials/Antibiotics"
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      "/Biology and life sciences/Organisms/Bacteria/Enterobacteriaceae/Escherichia/Escherichia coli"
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      "/Biology and life sciences/Organisms/Bacteria/Gut bacteria/Escherichia/Escherichia coli"
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    [
      "plos",
      "/Medicine and health sciences/Anatomy/Body fluids/Blood"
    ],
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      "plos",
      "/Medicine and health sciences/Health care/Health care facilities/Hospitals/Intensive care units"
    ],
    [
      "plos",
      "/Medicine and health sciences/Hematology/Bloodstream infections"
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      "/Medicine and health sciences/Pathology and laboratory medicine/Pathogens/Microbial pathogens/Bacterial pathogens/Escherichia coli"
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    [
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      "/Medicine and health sciences/Pathology and laboratory medicine/Pathogens/Microbial pathogens/Bacterial pathogens/Pseudomonas aeruginosa"
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      "/Research and analysis methods/Animal studies/Experimental organism systems/Model organisms/Escherichia coli"
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    [
      "plos",
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    [
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  "openalex": "W7212190555",
  "pdf_url": "https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0356199&type=printable",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-10",
  "publisher": "Public Library of Science",
  "ris": "https://research.draconitic.com/paper/0bem2gysfp91w.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:56Z",
      "native_id": "10.1371/journal.pone.0356199",
      "source": "plos",
      "url": "https://doi.org/10.1371/journal.pone.0356199"
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  "subjects": [
    {
      "field": "biology",
      "origin": "plos:microbiology",
      "subfield": "microbiology"
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    {
      "field": "medicine",
      "origin": "plos:anatomy",
      "subfield": "physiology"
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  "title": "Real-life evaluation of BioFire® BCID2 and Vitek® REVEALTM for Gram-negative bloodstream infections in critical care",
  "title_text": "Real-life evaluation of BioFire® BCID2 and Vitek® REVEALTM for Gram-negative bloodstream infections in critical care",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:56Z",
  "url": "https://research.draconitic.com/paper/0bem2gysfp91w",
  "venue": "PLOS One",
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}