{
  "abstract": "Targeted insertion of large DNA fragments has been constrained by the genotoxicity associated with double-strand breaks (DSBs) and the limitations of two-step recombinase systems. Three independently developed mammalian platforms, Quadruple pegRNA (QuadPE), Prime Assembly (PA), and PRIME-In, now achieve programmable, DSB- and recombinase-free insertion of up to 26 kb, 11.3 kb, and 9.2 kb, respectively, through convergent yet mechanistically distinct flap-guided integration strategies. Notably, QuadPE and PA achieve integration independent of cell-cycle progression. Furthermore, these systems outperform several existing recombinase- and/or transposase-based tools, underscoring their translational potential for therapeutic utility. Their mechanistic logic also provides a forward path for the DSB-free and recombinase-free integration of large DNA sequences in crop biotechnology.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Biotechnology, Indira Gandhi National Tribal University, Amarkantak, India."
  ],
  "article_type": "review article",
  "arxiv": null,
  "author_total": 2,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Koppolu Raja Rajesh Kumar"
    },
    {
      "affs": [
        0
      ],
      "name": "Ayushi Singh"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/0d6syz5ntt45r.bib",
  "cited_by": 0,
  "doi": "10.1016/j.bidere.2026.100126",
  "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13718414/?tool=EBI",
  "id": "0d6syz5ntt45r",
  "issns": [
    "2693-1257"
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  "landing_url": "https://europepmc.org/article/PMC/PMC13718414",
  "language": "eng",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Perspective"
    ],
    [
      "openalex",
      "1312|CRISPR and Genetic Engineering"
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  ],
  "openalex": "W7221313838",
  "pdf_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13718414/pdf/?tool=EBI",
  "pmcid": "PMC13718414",
  "pmid": "42960151",
  "published": "2026-10-10",
  "publisher": "Nanjing Agricultural University",
  "ris": "https://research.draconitic.com/paper/0d6syz5ntt45r.ris",
  "sources": [
    {
      "first_seen": "2026-10-31T00:20:38Z",
      "native_id": "PMC:PMC13718414",
      "source": "europepmc",
      "url": "https://europepmc.org/article/PMC/PMC13718414"
    },
    {
      "first_seen": "2026-11-01T00:20:52Z",
      "native_id": "MED:42960151",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42960151"
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    {
      "first_seen": "2026-11-02T12:22:26Z",
      "native_id": "PMC13718414",
      "source": "pmc",
      "url": "https://doi.org/10.1016/j.bidere.2026.100126"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|CRISPR and Genetic Engineering",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Precision meets scale: DSB- and recombinase-free targeted insertion of large DNA fragments",
  "title_text": "Precision meets scale: DSB- and recombinase-free targeted insertion of large DNA fragments",
  "type": "peer-reviewed",
  "updated_at": "2026-11-02T12:22:26Z",
  "url": "https://research.draconitic.com/paper/0d6syz5ntt45r",
  "venue": "Biodesign research",
  "versions": []
}