{
  "abstract": "Influenza A virus (IAV) remains a significant public health threat, with the need for effective vaccines and antiviral therapies remaining a high priority. In vitro testing is paramount in studying novel IAV viruses and their immunogenicity. To address this need, we developed a synthetic virology approach to rapidly prototype and test IAV mutants with altered antigenic sites. By strategically inserting restriction sites within an ambisense plasmid, we generated a tool where we can seamlessly replace either 1) the full hemagglutinin (HA), 2) the HA globular head domain to generate head/stalk chimeras or 3) the five canonical antigenic sites. The cloning inserts can be computationally designed and artificially synthesized, enabling rescue of synthetic Influenza A viruses. Using this platform we rescued IAV H1N1, H3N2 & H5N1, and went on to prototype their respective antigenic site mutants & chimeric IAVs using viral genome sequence alone. This platform could be useful for generating vaccine seed strains or to study anti-viral immunity.",
  "abstract_shown": true,
  "affiliations": [
    "University of Gothenburg"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 3,
  "authors": [
    {
      "name": "J. R. Murti"
    },
    {
      "name": "K. Schön"
    },
    {
      "affs": [
        0
      ],
      "name": "D. Angeletti"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/0h5mfkyzyqa32.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.10.24.761556",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.10.24.761556v1.full",
  "id": "0h5mfkyzyqa32",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.10.24.761556v1",
  "language": null,
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "synthetic biology"
    ]
  ],
  "openalex": null,
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.10.24.761556v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-24",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/0h5mfkyzyqa32.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:30:30Z",
      "native_id": "10.64898/2026.10.24.761556",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.10.24.761556v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:synthetic biology",
      "subfield": "systems-synthetic"
    }
  ],
  "title": "A Synthetic Virology Platform for Rapid Prototyping of Influenza A Virus mutants",
  "title_text": "A Synthetic Virology Platform for Rapid Prototyping of Influenza A Virus mutants",
  "type": "preprint",
  "updated_at": "2026-10-31T00:20:49Z",
  "url": "https://research.draconitic.com/paper/0h5mfkyzyqa32",
  "venue": "bioRxiv",
  "versions": []
}