{
  "abstract": "Small regulatory RNAs (sRNAs) are key players in bacterial adaptaFon to stress. They ofen occupy central posiFons in regulatory networks and control the expression of mulFple targets. In a striking example of this, the enterobacterial OmrA and OmrB paralogous sRNAs are known to regulate about ten different targets, with extensive data suggesFng the regulon is in fact much larger. Here we performed transcriptome and proteome analyses and idenFfied more than fifeen new targets of Escherichia coli OmrA and OmrB. We validated several, including genes involved in central carbon metabolism and fajy acid synthesis, among which ppc, actP and fabA. Consistent with a role in carbon metabolism, overproducing OmrA or OmrB inhibited growth on glucose minimal medium. The analysis of suppressor mutants shows that this is due to a decreased carbon flux through the TCA cycle. IncorporaFng other datasets such as RIL-seq, we generated a mulF-omics-based predicFon of target candidates. Together, our results show that OmrA/B base-pair to various regions of their mRNA targets, and therefore likely act through diverse regulatory mechanisms. Hence, this work extends the OmrA and OmrB regulons, establishes an unsuspected connecFon with carbon usage, and shows the benefits of combining global analyses to invesFgate sRNA regulons.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 10,
  "authors": [
    {
      "name": "Alexey Korepanov"
    },
    {
      "name": "Jonathan Jagodnik"
    },
    {
      "name": "Fanny Quenette"
    },
    {
      "name": "Thao Nguyen Le Lam"
    },
    {
      "name": "Marion Hamon"
    },
    {
      "name": "Jade Mathis de Fromont"
    },
    {
      "name": "Odile Sismeiro"
    },
    {
      "name": "Valentin Gherdol-Nouvion"
    },
    {
      "name": "Alexandre Maes"
    },
    {
      "name": "Maude Guillier"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/0jfmwh53w35kh.bib",
  "cited_by": 1,
  "doi": "10.64898/2026.06.26.734639",
  "fulltext_url": null,
  "id": "0jfmwh53w35kh",
  "issns": [],
  "landing_url": "https://hal.science/hal-05740914v1",
  "language": "en",
  "licence": "CC-BY-NC-ND-4.0",
  "licence_label": "CC BY-NC-ND 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "hal",
      "0.sdv"
    ],
    [
      "hal",
      "1.sdv.bbm"
    ],
    [
      "hal",
      "1.sdv.mp"
    ],
    [
      "hal",
      "2.sdv.bbm.gtp"
    ],
    [
      "hal",
      "2.sdv.mp.bac"
    ],
    [
      "openalex",
      "1311|Bacterial Genetics and Biotechnology"
    ]
  ],
  "openalex": "W7166458728",
  "pdf_url": "https://hal.science/hal-05740914/document",
  "pmcid": null,
  "pmid": null,
  "published": "2026-06-27",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/0jfmwh53w35kh.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:29Z",
      "native_id": "hal-05740914",
      "source": "hal",
      "url": "https://hal.science/hal-05740914v1"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "hal:2.sdv.bbm.gtp",
      "subfield": "proteomics-metabolomics"
    },
    {
      "field": "biology",
      "origin": "hal:1.sdv.mp",
      "subfield": "microbiology"
    }
  ],
  "title": "Studying the regulons of OmrA and OmrB paralogous small RNAs reveals targets involved in central carbon metabolism and lipogenesis",
  "title_text": "Studying the regulons of OmrA and OmrB paralogous small RNAs reveals targets involved in central carbon metabolism and lipogenesis",
  "type": "preprint",
  "updated_at": "2026-10-30T12:17:29Z",
  "url": "https://research.draconitic.com/paper/0jfmwh53w35kh",
  "venue": "HAL",
  "versions": []
}