{
  "abstract": "One run, two analytes. This RP–HPLC–DAD assay method was validated in accordance with ICH Q2(R2) criteria for the simple and robust quantification of minoxidil (MXD) and dexpanthenol (DXP) in topical pharmaceutical solutions. Utilizing a BDS Hypersil C18 column with an isocratic system— a 20 mM phosphate buffer (pH 2.5) and methanol in a 75:25 (v/v) ratio, respectively, pumped at a 1.0 mL/min— the setup achieved clear separation between MXD and DXP. Detection was carried out at 230 nm for MXD and 210 nm for DXP, yielding retention times of 11.34 and 4.09 min, respectively, with resolution of 6.65. The method exhibited good linearity over the concentration ranges of 75–500 µg/mL for MXD and 100–650 µg/mL for DXP, with R2 values of 0.9989 and 0.9988, respectively. The LOD and LOQ were 21.17 and 64.15 µg/mL for MXD and 25.71 and 77.92 µg/mL for DXP, respectively. The method demonstrated satisfactory accuracy and precision, with recoveries ranging from 98.22% to 100.46% for MXD and from 98.42% to 101.05% for DXP, while the relative standard deviation (%RSD) values remained below 2%. The proposed method was successfully applied to commercial topical solutions, demonstrating its practical utility for regular quality assurance workflows.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Pharmaceutical Chemistry & Quality Control, Faculty of Pharmacy, University of Aleppo, Aleppo, Syria",
    "Department of Biochemistry & Microbiology, Faculty of Pharmacy, University of Aleppo, Aleppo, Syria"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 4,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Aya Sabouny"
    },
    {
      "affs": [
        1
      ],
      "name": "Ali Ibrahim"
    },
    {
      "affs": [
        0
      ],
      "name": "Saleh Trefi"
    },
    {
      "affs": [
        0
      ],
      "name": "Yaser Bitar"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/0jssn81y0kg00.bib",
  "cited_by": 0,
  "doi": "10.21608/bfsa.2026.525514.3290",
  "fulltext_url": "https://bpsa.journals.ekb.eg/article_534835.html",
  "id": "0jssn81y0kg00",
  "issns": [
    "3009-7703",
    "1110-0052"
  ],
  "landing_url": "https://doi.org/10.21608/bfsa.2026.525514.3290",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "lcc",
      "HD9665-9675"
    ],
    [
      "lcc",
      "RM1-950"
    ],
    [
      "lcc",
      "RS1-441"
    ],
    [
      "openalex",
      "1602|Analytical Methods in Pharmaceuticals"
    ]
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  "openalex": "W7222896863",
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-25",
  "publisher": "Assiut University, Faculty of Pharmacy",
  "ris": "https://research.draconitic.com/paper/0jssn81y0kg00.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T13:45:15Z",
      "native_id": "d55a39e84ef643a59d9e39bbc753cfff",
      "source": "doaj",
      "url": "https://doi.org/10.21608/bfsa.2026.525514.3290"
    }
  ],
  "subjects": [
    {
      "field": "chemistry",
      "origin": "openalex:1602|Analytical Methods in Pharmaceuticals",
      "subfield": "analytical"
    }
  ],
  "title": "Simultaneous quantification of Minoxidil and Dexpanthenol in topical solutions: Development and validation of a RP-HPLC procedure",
  "title_text": "Simultaneous quantification of Minoxidil and Dexpanthenol in topical solutions: Development and validation of a RP-HPLC procedure",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T13:45:15Z",
  "url": "https://research.draconitic.com/paper/0jssn81y0kg00",
  "venue": "Bulletin of Pharmaceutical Sciences. Assiut University",
  "versions": []
}