{
  "abstract": "All four dengue virus serotypes (DENV-1 to DENV-4) can cause severe and potentially fatal disease. Understanding the interplay between host immune responses and viral genetic diversity is particularly crucial in hyperendemic regions, where serotype shifts frequently occur during outbreaks. In this cross-sectional, surveillance-based study, we analyzed 350 patients with confirmed secondary dengue infection in Lao PDR, with samples collected between 2022 and 2023. Of these, 80 were successfully serotyped by RT-PCR and included in the serotype-specific analyses. Viral genotyping was performed using envelope (E) gene sequencing, and neutralizing antibody (nAb) titers and antibody-dependent enhancement (ADE) activities against all four DENV serotypes were assessed using single-round infectious particles in both FcγRIIA-negative and FcγRIIA-positive BHK-21 cells. The nAb titers were highest against DENV-2, followed by DENV-1, DENV-4, and DENV-3. Nevertheless, DENV-2 exhibited the highest ADE activity, suggesting that high nAb titers may not necessarily prevent ADE. We also documented the co-circulation of DENV-2 Cosmopolitan and Asian I genotypes. Our findings highlight the complex interplay between neutralizing and enhancing antibody responses during secondary infection, particularly in the context of DENV-2 infection. Our results emphasize the need to understand the dynamics of DENV transmission in hyperendemic settings and may inform future vaccine development and evaluation strategies.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Virology, Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan",
    "Graduate School of Biomedical Sciences, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan",
    "Program for Nurturing Global Leaders in Tropical and Emerging Communicable Diseases, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan",
    "National Center for Laboratory and Epidemiology, Ministry of Health, Vientiane 01002, Laos",
    "Department of Communicable Diseases Control, Ministry of Health, Vientiane 01002, Laos",
    "School of Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan",
    "Department of Tropical Viral Vaccine Development, Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan",
    "Dejima Infectious Disease Research Alliance, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan",
    "Center for Vaccines Disease and Therapeutic Antibodies for Emerging Infectious Diseases, Shimane University, 1060 Nishikawatsucho, Matsue 690-8504, Japan",
    "Vietnam Research Station, Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 13,
  "authors": [
    {
      "affs": [
        0,
        1,
        2,
        3
      ],
      "name": "Dalouny Xayavong"
    },
    {
      "affs": [
        3
      ],
      "name": "Virasack Somoulay"
    },
    {
      "affs": [
        3
      ],
      "name": "Bouaphanh Khamphaphongphane"
    },
    {
      "affs": [
        4
      ],
      "name": "Phonepadith Xangxayarath"
    },
    {
      "affs": [
        0,
        5
      ],
      "name": "Hiromu Osako"
    },
    {
      "affs": [
        0,
        5
      ],
      "name": "Kei Yamasato"
    },
    {
      "affs": [
        0
      ],
      "name": "Motoki Ihara",
      "orcid": "0009-0004-9714-0698"
    },
    {
      "affs": [
        0,
        6
      ],
      "name": "Jean Claude Balingit",
      "orcid": "0000-0002-2165-907X"
    },
    {
      "affs": [
        0,
        6,
        7,
        8
      ],
      "name": "Mya Myat Ngwe Tun",
      "orcid": "0000-0002-8986-6307"
    },
    {
      "affs": [
        0,
        6,
        7
      ],
      "name": "Kouichi Morita",
      "orcid": "0000-0001-8391-1157"
    },
    {
      "affs": [
        0
      ],
      "name": "Arata Hidano",
      "orcid": "0000-0003-1707-5319"
    },
    {
      "affs": [
        1,
        9
      ],
      "name": "Futoshi Hasebe"
    },
    {
      "affs": [
        0,
        1,
        7
      ],
      "name": "Yuki Takamatsu",
      "orcid": "0009-0008-6706-438X"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/0kjdc3cr7f4bb.bib",
  "cited_by": 0,
  "doi": "10.3390/ijms27198869",
  "fulltext_url": "https://europepmc.org/articles/PMC13664945",
  "id": "0kjdc3cr7f4bb",
  "issns": [
    "1422-0067"
  ],
  "landing_url": "https://www.mdpi.com/1422-0067/27/19/8869",
  "language": "eng",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2739|Mosquito-borne diseases and control"
    ]
  ],
  "openalex": "W7220397831",
  "pdf_url": "https://europepmc.org/articles/PMC13664945?pdf=render",
  "pmcid": "PMC13664945",
  "pmid": "42884113",
  "published": "2026-10-05",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/0kjdc3cr7f4bb.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:55Z",
      "native_id": "10.3390/ijms27198869",
      "source": "crossref",
      "url": "https://www.mdpi.com/1422-0067/27/19/8869"
    },
    {
      "first_seen": "2026-10-30T12:19:00Z",
      "native_id": "MED:42884113",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42884113"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2739|Mosquito-borne diseases and control",
      "subfield": "epidemiology-public-health"
    }
  ],
  "title": "DENV-2 Genetic Diversity and Variation in Antibody-Dependent Enhancement in Lao PDR",
  "title_text": "DENV-2 Genetic Diversity and Variation in Antibody-Dependent Enhancement in Lao PDR",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:00Z",
  "url": "https://research.draconitic.com/paper/0kjdc3cr7f4bb",
  "venue": "International Journal of Molecular Sciences",
  "versions": []
}