{
  "abstract": "This work presents the development and validation of a candidate reference method for the simultaneous quantification of 3-nitro-L-tyrosine, 3-monoiodo-L-tyrosine, and 3,5-diiodo-L-tyrosine in urine. 13C and 15N labelled analogues of the three analytes were calibrated against natural abundance standards and used for the development of an isotope dilution quantification approach. Deuterated creatinine was also used to quantify in situ the creatinine content so that concentration of the target analytes relative to creatinine can be obtained in the same chromatogram. Liquid chromatography coupled to tandem mass spectrometry was used in the Selected Reaction Monitoring mode to measure isotopologue distributions from which concentration of the analytes are directly obtained without performing methodological calibrations. Chromatographic separation and mass spectrometric detection were optimized to achieve limits of quantification from 0.015 to 0.08 pmol center dot g-1. The full protocol was validated according to the Clinical and Laboratory Standards Institute (CLSI) guidelines, yielding satisfactory results. Interday variability (ranged from 0.8 to 2.5%) and intraday variability (from 0.7 to 4.2%) in levels of individuals with congenital hypothyroidism were evaluated. Accuracy was evaluated through recovery experiments in fortified urine obtaining 103 +/- 6, 97 +/- 4, 95 +/- 5% for 3-nitro-L-tyrosine, 3-monoiodo-L-tyrosine, and 3,5-diiodo-L-tyrosine, respectively. Linearity between 1.2 and 73.8, 1.8 and 102.3, and 0.6 and 35.8 pmol center dot g-1 for 3-NT, MIT, and DIT, respectively was obtained. Application to real samples was evaluated through the analysis of 1062 urine samples from pregnant women and their offspring. The candidate reference procedure demonstrated its robustness as a candidate reference procedure for the clinical investigation of thyroid hormone disorders and oxidative stress in the general population.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 9,
  "authors": [
    {
      "name": "Heli Potes-Rodriguez"
    },
    {
      "name": "Pablo Rodríguez-González"
    },
    {
      "name": "Juan Manuel Marchante-Gayon"
    },
    {
      "name": "Pouya Alikhani"
    },
    {
      "name": "Mònica Guxens"
    },
    {
      "name": "Anne-Claire Binter"
    },
    {
      "name": "Alireza Mani"
    },
    {
      "name": "Jose C Moreno"
    },
    {
      "name": "José Ignacio García‐alonso"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/0m3recbhverah.bib",
  "cited_by": 0,
  "doi": "10.1016/j.microc.2026.119391",
  "fulltext_url": null,
  "id": "0m3recbhverah",
  "issns": [
    "0026-265X",
    "1095-9149"
  ],
  "landing_url": "https://hal.science/hal-05768611v1",
  "language": "en",
  "licence": "CC-BY-NC-ND-4.0",
  "licence_label": "CC BY-NC-ND 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "hal",
      "0.sdv"
    ],
    [
      "openalex",
      "1602|Analytical Methods in Pharmaceuticals"
    ]
  ],
  "openalex": "W7202361509",
  "pdf_url": "https://hal.science/hal-05768611/document",
  "pmcid": null,
  "pmid": null,
  "published": "2026-08-13",
  "publisher": "Elsevier",
  "ris": "https://research.draconitic.com/paper/0m3recbhverah.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:01Z",
      "native_id": "hal-05768611",
      "source": "hal",
      "url": "https://hal.science/hal-05768611v1"
    }
  ],
  "subjects": [
    {
      "field": "chemistry",
      "origin": "openalex:1602|Analytical Methods in Pharmaceuticals",
      "subfield": "analytical"
    }
  ],
  "title": "Development and validation of a novel candidate reference procedure for the quantification of 3-nitro-L-tyrosine, 3-monoiodo-L-tyrosine and 3,5-diiodo-L-tyrosine in urine by isotope dilution and LC-ESI-MS/MS",
  "title_text": "Development and validation of a novel candidate reference procedure for the quantification of 3-nitro-L-tyrosine, 3-monoiodo-L-tyrosine and 3,5-diiodo-L-tyrosine in urine by isotope dilution and LC-ESI-MS/MS",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:18:01Z",
  "url": "https://research.draconitic.com/paper/0m3recbhverah",
  "venue": "Microchemical Journal",
  "versions": []
}