{
  "abstract": "To explore the constraints of forming consecutive C-AgI-T base pairs during DNA polymerase-catalyzed primer extension reactions, we examined the incorporation of deoxythymidine triphosphate (dTTP) into consecutive C sites in the presence of AgI ions. The results demonstrated that AgI-promoted primer extension enables the formation of up to three consecutive C-AgI-T base pairs. Importantly, suppression of C-AgI-C base pair formation resulting from dCTP incorporation was demonstrated to be essential for achieving this outcome. Furthermore, by using MnII ions, the formation of up to five consecutive C-AgI-T base pairs was achieved. The formation of consecutive T-AgI-C base pairs, which involves the incorporation of dCTP into templating T sites, is more difficult than the formation of consecutive C-AgI-T base pairs, because much higher concentrations of dTTP compete with the template thymine for the AgI ion that abstracts the N3 imino proton. Thermal denaturation studies demonstrated that Ag+ ions effectively stabilized duplexes containing consecutive C–T mismatches, whereas stabilization by consecutive C-Ag+-C base pairs was limited because of electrostatic repulsion between neighboring positively charged base pairs. These results suggest that charge-neutral and thermally stable metal-mediated base pairs are critical for efficient polymerase-catalyzed synthesis of metal-containing DNA. These insights could be beneficial for the enzymatic synthesis of DNA-based AgI nanowires.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "name": "Tatsuya Funai"
    },
    {
      "name": "Keiko Morita"
    },
    {
      "name": "Ayaka Tani"
    },
    {
      "name": "Shun-ichi Wada"
    },
    {
      "name": "Hidehito Urata"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/0m696fns05xgy.bib",
  "cited_by": 0,
  "doi": "10.1248/cpb.c26-00271",
  "fulltext_url": "https://www.jstage.jst.go.jp/article/cpb/74/10/74_c26-00271/_article",
  "id": "0m696fns05xgy",
  "issns": [
    "1347-5223",
    "0009-2363"
  ],
  "landing_url": "https://www.jstage.jst.go.jp/article/cpb/74/10/74_c26-00271/_article",
  "language": null,
  "licence": "CC-BY-NC",
  "licence_label": "CC BY-NC",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by-nc/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "jstage-journal",
      "chemistry/medicinal"
    ],
    [
      "openalex",
      "1312|DNA and Nucleic Acid Chemistry"
    ]
  ],
  "openalex": "W7223334682",
  "pdf_url": "https://www.jstage.jst.go.jp/article/cpb/74/10/74_c26-00271/_pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-24",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/0m696fns05xgy.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:34Z",
      "native_id": "10.1248/cpb.c26-00271",
      "source": "jstage",
      "url": "https://www.jstage.jst.go.jp/article/cpb/74/10/74_c26-00271/_article"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|DNA and Nucleic Acid Chemistry",
      "subfield": "molecular-biology"
    }
  ],
  "title": "DNA Polymerase Catalyzes Successive Formation of Cytosine-AgI-Thymine Base Pairs",
  "title_text": "DNA Polymerase Catalyzes Successive Formation of Cytosine-AgI-Thymine Base Pairs",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:34Z",
  "url": "https://research.draconitic.com/paper/0m696fns05xgy",
  "venue": "Chemical and Pharmaceutical Bulletin",
  "versions": []
}