{
  "abstract": "Transcription factor Ets2 coalesce with the NF-{kappa}B pathway to regulate gene expression in specific signaling contexts. IKK2/{beta}-mediated phosphorylation events critically regulate the NF-{kappa}B pathway. However, any link between Ets2 and IKK2 remains elusive. Here we report Ets2 as a direct substrate of IKK2. In-vitro kinase assays using deletion constructs, high resolution MS-MS and site directed mutagenesis identified S295 as a prominent phosphorylation site distal to the DNA binding domain, substitution of which to phosphor-mimetic Glutamate triggers further phosphorylation of Ets2. MD simulations clearly indicate conformational constriction of the otherwise disordered N-terminal region and inhibition of DNA binding activity upon phosphorylation, which was further confirmed by Electrophoretic mobility shift assays. Our results uncover a phosphoregulatory connection between Ets2 and IKK2.",
  "abstract_shown": true,
  "affiliations": [
    "Bose Institute"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "name": "D. Sutradhar"
    },
    {
      "name": "A. R. Antony"
    },
    {
      "name": "A. Haque"
    },
    {
      "name": "P. Borar"
    },
    {
      "name": "P. T. Rao"
    },
    {
      "name": "S. Raychaudhuri"
    },
    {
      "name": "P. Kumar"
    },
    {
      "affs": [
        0
      ],
      "name": "S. Polley"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/0n1prm9713hhm.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.08.31.747788",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.08.31.747788v1.full",
  "id": "0n1prm9713hhm",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.08.31.747788v1",
  "language": null,
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "biochemistry"
    ],
    [
      "openalex",
      "1306|NF-κB Signaling Pathways"
    ]
  ],
  "openalex": "W7204967590",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.08.31.747788v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-08-31",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/0n1prm9713hhm.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:11Z",
      "native_id": "10.64898/2026.08.31.747788",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.08.31.747788v1"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:1306|NF-κB Signaling Pathways",
      "subfield": "oncology"
    }
  ],
  "title": "IKK2/β mediated phosphorylation of transcription factor Ets2 at site(s) distal to DNA binding domain negatively modulates its DNA binding activity",
  "title_text": "IKK2/β mediated phosphorylation of transcription factor Ets2 at site(s) distal to DNA binding domain negatively modulates its DNA binding activity",
  "type": "preprint",
  "updated_at": "2026-10-30T12:18:11Z",
  "url": "https://research.draconitic.com/paper/0n1prm9713hhm",
  "venue": "bioRxiv",
  "versions": []
}