{
  "abstract": "Peptide-based fluorescent probes are valuable tools for live-cell imaging, but conventional labelling approaches often require washing or long incubation, limiting their signal-to-noise ratios. Fluorogenic amino acids (FgAAs) can overcome these limitations, yet their rational design remains challenging owing to the lack of generalizable quenching principles and synthetic routes compatible with diverse fluorophores. Here we developed a general strategy to convert constitutively bright fluorophores into turn-on FgAAs through intramolecular tryptophan-induced quenching. Computational analysis showed that quenching is governed by photoinduced electron transfer or twisted intramolecular charge transfer mechanisms, with adaptability across ultraviolet-to-near-infrared fluorophore scaffolds. We synthesized a large library of multicolour FgAAs and integrated them into solid-phase peptide synthesis to generate turn-on probes for real-time microscopy in live cells. We then designed fluorogenic peptides targeting programmed cell death protein 1 (PD-1), enabling T cell targeted drug screening and identifying avasimibe as a small-molecule modulator of PD-1 activity. This work establishes a rational strategy for designing multicolour FgAAs for peptide engineering, imaging and drug discovery.",
  "abstract_shown": true,
  "affiliations": [
    "Centre for Inflammation Research, Institute for Regeneration and Repair, The University of Edinburgh, Edinburgh, UK",
    "IRR Chemistry Hub, Institute for Regeneration and Repair, The University of Edinburgh, Edinburgh, UK",
    "Fluorescence Research Group, Singapore University of Technology and Design, Singapore, Singapore",
    "Concept Life Sciences Ltd, Edinburgh Bioquarter, Edinburgh, UK",
    "Department of Applied Chemistry, Graduate School of Engineering, The University of Osaka, Suita, Japan",
    "School of Chemistry, Chemical Engineering and Biotechnology, Nanyang Technological University, Singapore, Singapore"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 11,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Man Sing Wong"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Lorena Mendive-Tapia",
      "orcid": "0000-0002-3406-5203"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Utsa Karmakar",
      "orcid": "0000-0001-5323-3500"
    },
    {
      "affs": [
        2
      ],
      "name": "Lovelesh Vashist"
    },
    {
      "affs": [
        3
      ],
      "name": "Zandile Nare"
    },
    {
      "affs": [
        3
      ],
      "name": "Karolina Tokarczyk",
      "orcid": "0000-0002-7157-3581"
    },
    {
      "affs": [
        4
      ],
      "name": "Kohei Iijima"
    },
    {
      "affs": [
        4
      ],
      "name": "Kazuya Kikuchi"
    },
    {
      "affs": [
        2,
        5
      ],
      "name": "Syed Ali Abbas Abedi",
      "orcid": "0000-0001-6131-5039"
    },
    {
      "affs": [
        2,
        5
      ],
      "name": "Xiaogang Liu",
      "orcid": "0000-0002-2553-2068"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Marc Vendrell",
      "orcid": "0000-0002-5392-9740"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/0nrrxwf06srsr.bib",
  "cited_by": 0,
  "doi": "10.1038/s41557-026-02228-0",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13719488/",
  "id": "0nrrxwf06srsr",
  "issns": [
    "1755-4330",
    "1755-4349"
  ],
  "landing_url": "https://doi.org/10.1038/s41557-026-02228-0",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Article"
    ],
    [
      "openalex",
      "1312|Chemical Synthesis and Analysis"
    ]
  ],
  "openalex": "W7204256762",
  "pdf_url": "https://www.nature.com/articles/s41557-026-02228-0.pdf",
  "pmcid": "PMC13719488",
  "pmid": "42649276",
  "published": "2026-08-26",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/0nrrxwf06srsr.ris",
  "sources": [
    {
      "first_seen": "2026-11-02T12:23:26Z",
      "native_id": "PMC13719488",
      "source": "pmc",
      "url": "https://doi.org/10.1038/s41557-026-02228-0"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Chemical Synthesis and Analysis",
      "subfield": "molecular-biology"
    }
  ],
  "title": "A general strategy towards multicolour fluorogenic peptides for wash-free bioassays",
  "title_text": "A general strategy towards multicolour fluorogenic peptides for wash-free bioassays",
  "type": "peer-reviewed",
  "updated_at": "2026-11-03T12:20:32Z",
  "url": "https://research.draconitic.com/paper/0nrrxwf06srsr",
  "venue": "Nature Chemistry",
  "versions": []
}