{
  "abstract": "Severe fever with thrombocytopenia syndrome (SFTS), caused by Dabie bandavirus (DBV), has rapidly spread to multiple countries worldwide since its discovery in 2009, posing a serious threat to global public health security. SFTS is a tick-borne disease, and people living in mountainous and hilly areas are at increased risk of infection, which highlights the urgent need for rapid, accurate, and field-applicable diagnostic strategies for DBV. In this study, LAMP primers and CRISPR sgRNA were designed based on the conserved region of the DBV L segment. A one-pot, one-step molecular assay integrating reverse transcription loop-mediated isothermal amplification (RT-LAMP) with CRISPR/Cas12b technology was established after systematic optimization of key parameters. The assay operates under isothermal conditions at 60 °C, delivering results within 40 min. Analytical evaluation demonstrated a limit of detection of 25.6 RNA copies per reaction. No cross-reactivity was observed with Hantaan virus, Seoul virus, Chikungunya virus, Japanese encephalitis virus, Dengue virus, and Zika virus. Clinical validation using 42 specimens showed complete concordance with gold-standard qRT-PCR, achieving 100% sensitivity (30/30) and 100% specificity (12/12). For point-of-care application, fluorescence visualization was incorporated into the CRISPR/Cas-based detection workflow, enabling straightforward result interpretation. This integrated RT-LAMP-CRISPR/Cas12b platform provides rapid and specific detection of DBV through a simplified workflow that does not require complex instrumentation, supporting its potential application in resource-limited environments.",
  "abstract_shown": true,
  "affiliations": [
    "Jiangsu Provincial Center for Disease Control and Prevention , , ,"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Jinlei Guo",
      "orcid": "0009-0006-6695-8205"
    },
    {
      "affs": [
        0
      ],
      "name": "Tao Wu"
    },
    {
      "affs": [
        0
      ],
      "name": "Qiao Qiao"
    },
    {
      "affs": [
        0
      ],
      "name": "Xiaojuan Zhu"
    },
    {
      "affs": [
        0
      ],
      "name": "Kangchen Zhao"
    },
    {
      "affs": [
        0
      ],
      "name": "Lunbiao Cui"
    },
    {
      "affs": [
        0
      ],
      "name": "Xiang Huo"
    },
    {
      "affs": [
        0
      ],
      "name": "Yiyue Ge"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/0pczk5a16vfsg.bib",
  "cited_by": 0,
  "doi": "10.1021/acsomega.6c05919",
  "fulltext_url": "https://doi.org/10.1021/acsomega.6c05919",
  "id": "0pczk5a16vfsg",
  "issns": [
    "2470-1343"
  ],
  "landing_url": "https://pubs.acs.org/acsodf/article/doi/10.1021/acsomega.6c05919/5455924/Development-of-a-One-Step-RT-LAMP-CRISPR-Cas12b",
  "language": "en",
  "licence": "CC-BY-NC-ND-4.0",
  "licence_label": "CC BY-NC-ND 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "lcc",
      "QD1-999"
    ],
    [
      "openalex",
      "2204|Biosensors and Analytical Detection"
    ]
  ],
  "openalex": "W7223172316",
  "pdf_url": "https://pubs.acs.org/doi/pdf/10.1021/acsomega.6c05919",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-01",
  "publisher": "American Chemical Society (ACS)",
  "ris": "https://research.draconitic.com/paper/0pczk5a16vfsg.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:39Z",
      "native_id": "10.1021/acsomega.6c05919",
      "source": "crossref",
      "url": "https://pubs.acs.org/acsodf/article/doi/10.1021/acsomega.6c05919/5455924/Development-of-a-One-Step-RT-LAMP-CRISPR-Cas12b"
    },
    {
      "first_seen": "2026-11-04T14:20:38Z",
      "native_id": "052e2290445c459da097602f390cd9e0",
      "source": "doaj",
      "url": "https://doi.org/10.1021/acsomega.6c05919"
    }
  ],
  "subjects": [
    {
      "field": "chemistry",
      "origin": "lcc:QD1-999",
      "subfield": "general"
    }
  ],
  "title": "Development of a One-Step RT-LAMP-CRISPR/Cas12b Assay for Rapid On-Site Detection of Dabie Bandavirus",
  "title_text": "Development of a One-Step RT-LAMP-CRISPR/Cas12b Assay for Rapid On-Site Detection of Dabie Bandavirus",
  "type": "peer-reviewed",
  "updated_at": "2026-11-05T14:20:34Z",
  "url": "https://research.draconitic.com/paper/0pczk5a16vfsg",
  "venue": "ACS Omega",
  "versions": []
}