{
  "abstract": "The epidermal growth factor receptor (EGFR) and hepatocyte growth factor receptor (c-Met/MET) are receptor tyrosine kinases (RTKs) whose signalling depends on endocytic routing, and impaired down-regulation can drive tumour progression and therapy resistance, including MET-driven bypass of EGFR inhibition. However, how routing states couple to signalling and lipid programs, and how this differs between receptors under matched conditions, remains unclear. Using a chemically diverse annotated inhibitor library, we performed time-resolved, pathway-focused high-content imaging in A549 cells to compare co-varying phenotypes across matched EGFR and c-Met assay systems. Late receptor persistence defined operational degradation-inhibitor phenotypes that were heterogeneous and dispersed across a fused phenotypic atlas. Despite this heterogeneity, matched degradation-inhibitor sets showed receptor-selective lipid–trafficking coupling: EGFR persistence was associated with reduced phosphatidylinositol 4,5-bisphosphate-linked readouts and signalling biases, whereas c-Met persistence aligned with increased perinuclear phosphatidylinositol 4-phosphate heterogeneity and altered transferrin-recycling readouts. These results provide a comparative imaging framework for mapping RTK trafficking states and nominate phosphoinositide-linked features as candidate readouts of trafficking rewiring in cancer-relevant contexts.",
  "abstract_shown": true,
  "affiliations": [
    "Institute for Comprehensive Medical Sciences, Tokyo Women’s Medical University"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 1,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Kenji Tanabe"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/0qc763q0pbz33.bib",
  "cited_by": 0,
  "doi": "10.1038/s41598-026-61308-w",
  "fulltext_url": "https://doi.org/10.1038/s41598-026-61308-w",
  "id": "0qc763q0pbz33",
  "issns": [
    "2045-2322"
  ],
  "landing_url": "https://doi.org/10.1038/s41598-026-61308-w",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "lcc",
      "Q"
    ],
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      "lcc",
      "R"
    ],
    [
      "openalex",
      "1307|Cellular transport and secretion"
    ]
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  "pdf_url": "https://www.nature.com/articles/s41598-026-61308-w_reference.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-07-01",
  "publisher": "Nature Portfolio",
  "ris": "https://research.draconitic.com/paper/0qc763q0pbz33.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T13:45:52Z",
      "native_id": "1926077c79d34aefbcdc2f415dbc6c6f",
      "source": "doaj",
      "url": "https://doi.org/10.1038/s41598-026-61308-w"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1307|Cellular transport and secretion",
      "subfield": "cell-biology"
    }
  ],
  "title": "Time-resolved phenotypic profiling maps EGFR and c-Met trafficking signatures",
  "title_text": "Time-resolved phenotypic profiling maps EGFR and c-Met trafficking signatures",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T13:45:52Z",
  "url": "https://research.draconitic.com/paper/0qc763q0pbz33",
  "venue": "Scientific Reports",
  "versions": []
}