{
  "abstract": "To develop sustainable extraction of bioactive compounds, this study evaluated the potential of betaine-based deep eutectic solvents (DES). Betaine was applied as a natural, safe hydrogen-bond acceptor in fourteen DES, using polyols/sugars/acids as hydrogen-bond donors to extract vine shoots (VS) phenolic compounds. A conventional solvent, 59% (v/v) ethanol/water, was used for comparison. Among the tested DES, the extracts obtained with betaine:ethylene glycol:water (1:3:5) and betaine:sorbitol:water (3:1:10) demonstrated high extraction capacity. The betaine:ethylene glycol:water (1:3:5) DES yielded the extract with the most favourable biocompatibility profile and adequate stability at room temperature. The extraction of VS with this DES was optimised using response surface methodology, varying temperature, time, and solid load. It enabled an increase of both the total phenolic content (up to 2.35 mg GAE/mL) and the antioxidant activity (EC₅₀ of 162.5 mg extract/mL) under 80 °C, 127 min, and L/S 10:1. Moreover, the DES extract was further profiled by HPLC-UV and HPLC-QTOF-MS, highlighting the occurrence of the high-demand antioxidant trans-resveratrol (16 µg/mL) and its dimer trans-ε-viniferin (31 µg/mL). To the best of our knowledge, this is the first study to evaluate betaine-based DES for VS extraction, along with its characterisation and cytotoxicity assessment, while discussing their limitations.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Chemical, Environmental and Materials Engineering, Universidad de Jaén, 23071 Jaén, Spain.",
    "Institute of Biorefineries Research (I3B), Universidad de Jaén, 23071 Jaén, Spain.",
    "LAQV, REQUIMTE, Departamento de Química da Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Camila C Muñoz-Realpe",
      "orcid": "0000-0003-2674-8212"
    },
    {
      "affs": [
        2
      ],
      "name": "Carolina Costa",
      "orcid": "0009-0008-2610-4621"
    },
    {
      "affs": [
        2
      ],
      "name": "Ana Rita Jesus",
      "orcid": "0000-0002-0840-6289"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "María Del Mar Contreras",
      "orcid": "0000-0002-3407-0088"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Inmaculada Romero",
      "orcid": "0000-0002-4152-8034"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Eulogio Castro",
      "orcid": "0000-0003-1719-6049"
    },
    {
      "affs": [
        2
      ],
      "name": "Ana Rita C Duarte"
    },
    {
      "affs": [
        2
      ],
      "name": "Alexandre Paiva"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/0qzfaksjt7fj8.bib",
  "cited_by": 0,
  "doi": "10.3390/foods15203708",
  "fulltext_url": "https://europepmc.org/articles/PMC13704195",
  "id": "0qzfaksjt7fj8",
  "issns": [
    "2304-8158"
  ],
  "landing_url": "https://europepmc.org/article/MED/42940345",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1503|Ionic liquids properties and applications"
    ]
  ],
  "openalex": "W7222944790",
  "pdf_url": "https://europepmc.org/articles/PMC13704195?pdf=render",
  "pmcid": "PMC13704195",
  "pmid": "42940345",
  "published": "2026-10-18",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/0qzfaksjt7fj8.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:22:12Z",
      "native_id": "MED:42940345",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42940345"
    }
  ],
  "subjects": [
    {
      "field": "chemistry",
      "origin": "openalex:1503|Ionic liquids properties and applications",
      "subfield": "catalysis"
    }
  ],
  "title": "Betaine-Based Deep Eutectic Solvents for Phenolic Compounds Extraction from Vine Shoots: Screening, Optimisation, and Cytotoxicity Assessment.",
  "title_text": "Betaine-Based Deep Eutectic Solvents for Phenolic Compounds Extraction from Vine Shoots: Screening, Optimisation, and Cytotoxicity Assessment.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:22:12Z",
  "url": "https://research.draconitic.com/paper/0qzfaksjt7fj8",
  "venue": "Foods (Basel, Switzerland)",
  "versions": []
}