{
  "abstract": "Adhesion of Caenorhabditis elegans to plastic pipette tips and microcentrifuge tubes is a common source of sample loss and variability in experimental workflows. Here, we describe a simple consumable-preparation method using 0.5% Pluronic F-127 to reduce worm sticking and improve recovery from plastic surfaces. As a less hazardous alternative to traditional siliconization procedures, Pluronic-treated tips and tubes enabled consistent worm release, while increasing the concentration to 1% provided no additional benefit. Recovery efficiency was not retained following autoclaving of treated consumables. This protocol offers a practical and accessible approach to improving reproducibility in C. elegans handling and sample-processing workflows.",
  "abstract_shown": true,
  "affiliations": [
    "Biochemistry and Microbiology, Oklahoma State University Center for Health Sciences, Tulsa, OK, United States."
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 2,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Crystal A Shults"
    },
    {
      "affs": [
        0
      ],
      "name": "Jacob Manjarrez"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/0r17bwah6naxh.bib",
  "cited_by": 0,
  "doi": "10.17912/micropub.biology.002332",
  "fulltext_url": "https://europepmc.org/articles/PMC13743996",
  "id": "0r17bwah6naxh",
  "issns": [
    "2578-9430"
  ],
  "landing_url": "https://europepmc.org/article/MED/42999579",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2204|Microfluidic and Capillary Electrophoresis Applications"
    ]
  ],
  "openalex": "W7223246572",
  "pdf_url": "https://europepmc.org/articles/PMC13743996?pdf=render",
  "pmcid": "PMC13743996",
  "pmid": "42999579",
  "published": "2026-10-22",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/0r17bwah6naxh.ris",
  "sources": [
    {
      "first_seen": "2026-11-09T15:20:41Z",
      "native_id": "MED:42999579",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42999579"
    }
  ],
  "subjects": [],
  "title": "A Simple Pluronic F-127 Pretreatment Enables Near-Complete Recovery of C. elegans from Plastic Pipette Tips and Microcentrifuge Tubes.",
  "title_text": "A Simple Pluronic F-127 Pretreatment Enables Near-Complete Recovery of C. elegans from Plastic Pipette Tips and Microcentrifuge Tubes.",
  "type": "peer-reviewed",
  "updated_at": "2026-11-09T15:20:41Z",
  "url": "https://research.draconitic.com/paper/0r17bwah6naxh",
  "venue": "MicroPublication biology",
  "versions": []
}