{
  "abstract": "Gastric Cancer Stem Cells (GCSCs) contribute to tumor progression and treatment resistance, but their heterogeneity and tumor microenvironment interactions remain unclear. We analyzed GSE163558 single-cell RNA-sequencing data from 10 gastric cancer samples and integrated GCSC-related genes with The Cancer Genome Atlas Stomach Adenocarcinoma (TCGA-STAD) training cohort (n = 448) and two independent external validation cohorts, GSE62254 (n = 300) and GSE84437 (n = 483), to construct and validate a six-gene prognostic risk model. Cell–cell communication, metabolic activity, immune infiltration, immunotherapy response, and drug sensitivity were evaluated. Nine cell populations, including a putative GCSC population, were identified. GCSCs showed higher inferred oxidative phosphorylation activity and extensive predicted interactions with tumor-associated cells. The model stratified patients into distinct prognostic groups. High-risk tumors exhibited enrichment of progression-related pathways, including epithelial–mesenchymal transition, and distinct immune features. In PRJEB25780 (n = 78), higher risk scores were associated with immunotherapy non-response, whereas an exploratory analysis of GSE91061 (n = 49) showed a similar trend. The model may support prognostic stratification and provide insights into potential associations with therapeutic response, pending prospective validation.",
  "abstract_shown": true,
  "affiliations": [
    "College of Bioinformatics Science and Technology, Harbin Medical University, Harbin 150081, China"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 9,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Xin Hu"
    },
    {
      "affs": [
        0
      ],
      "name": "Shiqi Fan"
    },
    {
      "affs": [
        0
      ],
      "name": "Naiqiang Liu",
      "orcid": "0009-0008-5685-145X"
    },
    {
      "affs": [
        0
      ],
      "name": "Muchen Fan",
      "orcid": "0009-0000-4641-8486"
    },
    {
      "affs": [
        0
      ],
      "name": "Yewen Wu"
    },
    {
      "affs": [
        0
      ],
      "name": "Guang Shi"
    },
    {
      "affs": [
        0
      ],
      "name": "Yangguang Su"
    },
    {
      "affs": [
        0
      ],
      "name": "Lei Liu"
    },
    {
      "affs": [
        0
      ],
      "name": "Xiujie Chen",
      "orcid": "0000-0003-2423-8569"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/0rj38nhfky39d.bib",
  "cited_by": 0,
  "doi": "10.3390/ijms27219669",
  "fulltext_url": null,
  "id": "0rj38nhfky39d",
  "issns": [
    "1422-0067"
  ],
  "landing_url": "https://www.mdpi.com/1422-0067/27/21/9669",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2740|Gastric Cancer Management and Outcomes"
    ]
  ],
  "openalex": "W7224124659",
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-28",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/0rj38nhfky39d.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:58Z",
      "native_id": "10.3390/ijms27219669",
      "source": "crossref",
      "url": "https://www.mdpi.com/1422-0067/27/21/9669"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2740|Gastric Cancer Management and Outcomes",
      "subfield": "general"
    }
  ],
  "title": "Construction of a GCSCs-Related Prognostic Risk Model Based on Transcriptomic Data",
  "title_text": "Construction of a GCSCs-Related Prognostic Risk Model Based on Transcriptomic Data",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:18:58Z",
  "url": "https://research.draconitic.com/paper/0rj38nhfky39d",
  "venue": "International Journal of Molecular Sciences",
  "versions": []
}