{
  "abstract": "Background There is no licensed specific vaccine against the current Ebola Bundibugyo virus (BDBV) outbeak affecting the Democratic Republic of the Congo. However, clinical evaluations of a licensed Zaire ebolavirus (EBOV) vaccine have just launched. It remains unclear whether EBOV infection or vaccination generate cross-reactive immunity against BDBV. Methods We quantified CD4 and CD8 T-cell magnitude, polyfunctionality, and cytotoxicity after 8-day expansion with EBOV, BDBV, Sudan (SUDV), or Marburg (MARV) GP peptide pools, and measured GP-binding antibodies by multiplex assay, in recipients of Ad26.ZEBOV/MVA-BN-Filo (n=10), single-dose rVSVG-ZEBOV-GP (n=10), or two-dose rVSVG-ZEBOV-GP (n=8) (NCT02876328), and in EVD survivors sampled approximately 3 years after infection (n=10). Findings Fourteen days after completion of each licensed vaccine regimen, median BDBV-reactive CD4 responses were 9.8% [IQR:8.9-14.0] after single-dose rVSVG-ZEBOV-GP (D14) and 8.0% [5.5-9.9] after Ad26.ZEBOV/MVA-BN-Filo (D70). The highest BDBV-reactive CD8response was observed in survivors (24.7% [20.3-33.1]), together with a prominent cytotoxic CD8 response (14.4% [10.4-19.7]) ; corresponding vaccine-group medians ranged from 0.9% to 17.7% and from 0.6% to 6.7%, respectively. SUDV responses were also detected, whereas MARV recognition was limited. BDBV-binding antibodies were detected following both vaccine regimens, with the highest levels observed in survivors (491 [330-1261] net MFI). Among vaccinees, EBOV- and BDBV-reactive cellular and humoral responses were positively correlated after adjustment for multiple testing. Interpretation EBOV infection and vaccination elicited BDBV-cross-reactive T-cell responses and binding antibodies. These findings support further evaluation of existing EBOV vaccines in the context of BDBV outbreaks. Funding INSERM, Vaccine Research Institute (ANR-10-LABX-77-01), and Institut de Recherche pour le Developpement (IRD).",
  "abstract_shown": true,
  "affiliations": [
    "Vaccine Research Institute"
  ],
  "article_type": "PUBLISHAHEADOFPRINT",
  "arxiv": null,
  "author_total": 11,
  "authors": [
    {
      "name": "A. Wiedemann"
    },
    {
      "name": "A. Ayouba"
    },
    {
      "name": "E. Lhomme"
    },
    {
      "name": "E. Foucat"
    },
    {
      "name": "S. Kisrani"
    },
    {
      "name": "G. Thaurignac"
    },
    {
      "name": "S. Ben-Farhat"
    },
    {
      "name": "Postebogui Study group"
    },
    {
      "name": "PREVAC study team"
    },
    {
      "name": "M. Peeters"
    },
    {
      "affs": [
        0
      ],
      "name": "Y. Levy"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/0s0w628agjz11.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.11.03.26367168",
  "fulltext_url": "https://www.medrxiv.org/content/10.64898/2026.11.03.26367168v1.full",
  "id": "0s0w628agjz11",
  "issns": [],
  "landing_url": "https://www.medrxiv.org/content/10.64898/2026.11.03.26367168v1",
  "language": null,
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "medrxiv",
      "infectious diseases"
    ]
  ],
  "openalex": null,
  "pdf_url": "https://www.medrxiv.org/content/10.64898/2026.11.03.26367168v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-11-03",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/0s0w628agjz11.ris",
  "sources": [
    {
      "first_seen": "2026-11-05T00:20:40Z",
      "native_id": "10.64898/2026.11.03.26367168",
      "source": "medrxiv",
      "url": "https://www.medrxiv.org/content/10.64898/2026.11.03.26367168v1"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "medrxiv:infectious diseases",
      "subfield": "infectious-disease"
    }
  ],
  "title": "Cross-reactive antibody and functional T-cell responses to Bundibugyo virus induced by Zaire ebolavirus vaccination and infection",
  "title_text": "Cross-reactive antibody and functional T-cell responses to Bundibugyo virus induced by Zaire ebolavirus vaccination and infection",
  "type": "preprint",
  "updated_at": "2026-11-06T00:20:55Z",
  "url": "https://research.draconitic.com/paper/0s0w628agjz11",
  "venue": "medRxiv",
  "versions": []
}