{
  "abstract": "Purpose: This study aimed at gaining new insights into the pathophysiology of keratoconus by providing a comprehensive and quantitative characterization of stromal structural alterations throughout the full thickness of keratoconic corneas and in regions both proximal to and distant from the cone.\n\nMethods: Complementary three-dimensional imaging techniques were combined in a pilot exploratory series of four keratoconic and four control human corneas: clinical spectral-domain optical coherence tomography, highly-resolved line-field confocal optical coherence tomography and second harmonic generation (SHG) microcopy. Several structural metrics were computed from SHG images using automatic image analysis pipelines.\n\nResults: Quantitative SHG imaging revealed striking structural shifts in keratoconic corneas compared to healthy controls. Most notably, we observed a significant rise in normalized striation Endpoints (P = 0.008) and End/Branch ratio (P = 0.015), consistent with the presence of wavy lamellae and the loss of the plywood-like structure found in control corneas. Importantly, these structural markers remained consistent in both the cone and outside the cone regions. Quantitative processing of collagen orientation revealed a single widely-spread orientation as opposed to two main orthogonal orientations in control corneas, and slight shifts in orientation between superimposed lamellae rather than the abrupt changes observed in control corneas.\n\nConclusions: We provide new quantitative data on the structural alterations associated with keratoconus that could serve as useful biomarkers for earlier detection. Our findings are consistent with a decreased stromal stiffness and an increase in the corneal birefringence. They are also in favor of a global stromal disorder rather than a purely localized disorder.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "name": "Poncia Nyembo Kasongo"
    },
    {
      "name": "Djida Ghoubay"
    },
    {
      "name": "Jean-Marc Allain"
    },
    {
      "name": "Malika Hamrani"
    },
    {
      "name": "Cristina Georgeon"
    },
    {
      "name": "Vincent Borderie"
    },
    {
      "name": "Gaël Latour"
    },
    {
      "name": "Marie-Claire Schanne-Klein"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/0sgsx00ncm1wx.bib",
  "cited_by": 0,
  "doi": "10.1167/iovs.67.12.58",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13715923/",
  "id": "0sgsx00ncm1wx",
  "issns": [
    "0146-0404",
    "1552-5783"
  ],
  "landing_url": "https://hal.science/hal-05838438v1",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "hal",
      "0.phys"
    ],
    [
      "hal",
      "0.sdv"
    ],
    [
      "hal",
      "0.spi"
    ],
    [
      "hal",
      "1.phys.phys"
    ],
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      "hal",
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      "hal",
      "1.sdv.mhep"
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      "1.spi.opti"
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    [
      "hal",
      "2.sdv.mhep.os"
    ],
    [
      "heading",
      "Cornea"
    ],
    [
      "openalex",
      "2741|Corneal surgery and disorders"
    ]
  ],
  "openalex": "W7223357609",
  "pdf_url": "https://hal.science/hal-05838438/document",
  "pmcid": "PMC13715923",
  "pmid": "42924991",
  "published": "2026-10-23",
  "publisher": "Association for Research in Vision and Ophthalmology",
  "ris": "https://research.draconitic.com/paper/0sgsx00ncm1wx.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:01Z",
      "native_id": "hal-05838438",
      "source": "hal",
      "url": "https://hal.science/hal-05838438v1"
    },
    {
      "first_seen": "2026-11-01T12:23:06Z",
      "native_id": "PMC13715923",
      "source": "pmc",
      "url": "https://doi.org/10.1167/iovs.67.12.58"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "hal:1.sdv.ib",
      "subfield": "systems-synthetic"
    },
    {
      "field": "medicine",
      "origin": "hal:2.sdv.ib.ima",
      "subfield": "imaging"
    },
    {
      "field": "physics",
      "origin": "hal:2.phys.phys.phys-bio-ph",
      "subfield": "biological-medical"
    }
  ],
  "title": "Quantitation of stromal distortion across the full depth of keratoconic corneas",
  "title_text": "Quantitation of stromal distortion across the full depth of keratoconic corneas",
  "type": "peer-reviewed",
  "updated_at": "2026-11-01T12:23:06Z",
  "url": "https://research.draconitic.com/paper/0sgsx00ncm1wx",
  "venue": "Investigative Ophthalmology & Visual Science",
  "versions": []
}