{
  "abstract": "Ectopic expression of NGN1, BRN3A and ISLET1 (NBI) from a safe harbor locus in induced pluripotent stem cells (iPSCs) yielded robust differentiation into functional sensory neurons (iNBI-SNs) within seven days. Single nucleus transcriptomics identified peripheral sensory neuron profiles of nociceptors and mechanoreceptors. Electrophysiological studies showed that more than 98 % of iNBI-SNs display TTX-resistant sodium currents and that they establish functional connections to excitatory CNS neurons. iNBI-SNs derived from patients with inherited erythromelalgia, a pain disorder associated with gain-of-function mutations in the Nav1.7 sodium channel showed pathologically increased firing rates which could be partially rescued with a Nav1.7 inhibitor. Notably, iNBI-SNs acquire a characteristic pseudounipolar morphology upon co-culture with embryonic rodent DRG cells. Taken together, NBI-based forward programming of iPSCs represents a robust approach for the generation of human sensory neurons suitable for developmental, disease- and therapy-related studies.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 26,
  "authors": [
    {
      "name": "P. Röderer"
    },
    {
      "name": "A. C. Costa"
    },
    {
      "name": "M. Lürkens"
    },
    {
      "name": "F. M. Roll"
    },
    {
      "name": "F. Buck"
    },
    {
      "name": "L. Heidrich"
    },
    {
      "name": "J. Aicher"
    },
    {
      "name": "A. Neureiter"
    },
    {
      "name": "O. Mossad"
    },
    {
      "name": "H. Trucks-Jansen"
    },
    {
      "name": "K. Le Cann"
    },
    {
      "name": "A. K. Kalia"
    },
    {
      "name": "R. Balaji"
    },
    {
      "name": "V. Arndt"
    },
    {
      "name": "A. Zellner"
    },
    {
      "name": "Y. Mecdad"
    },
    {
      "name": "T. Bajaj"
    },
    {
      "name": "M. Stüger"
    },
    {
      "name": "M. K. Schwarz"
    },
    {
      "name": "A. Nitzsche"
    },
    {
      "name": "N. C. Gassen"
    },
    {
      "name": "N. Haag"
    },
    {
      "name": "M. Schröter"
    },
    {
      "name": "A. Lampert"
    },
    {
      "name": "M. M. Sousa"
    },
    {
      "name": "O. Brüstle"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/0sp7nrrje5ggc.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.09.18.747563",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.09.18.747563v1.full",
  "id": "0sp7nrrje5ggc",
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  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.09.18.747563v1",
  "language": null,
  "licence": "CC-BY-NC",
  "licence_label": "CC BY-NC",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "neuroscience"
    ],
    [
      "openalex",
      "1312|Pluripotent Stem Cells Research"
    ]
  ],
  "openalex": "W7213951594",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.09.18.747563v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-18",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/0sp7nrrje5ggc.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:23:29Z",
      "native_id": "10.64898/2026.09.18.747563",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.09.18.747563v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:neuroscience",
      "subfield": "neuroscience"
    }
  ],
  "title": "In vitro programming and pseudounipolarization of human iPSC-derived sensory neurons",
  "title_text": "In vitro programming and pseudounipolarization of human iPSC-derived sensory neurons",
  "type": "preprint",
  "updated_at": "2026-10-30T12:23:29Z",
  "url": "https://research.draconitic.com/paper/0sp7nrrje5ggc",
  "venue": "bioRxiv",
  "versions": []
}