{
  "abstract": "In eukaryotes, catalases are peroxisomal antioxidant enzymes that maintain cellular reactive oxygen species (ROS) homeostasis. Plant catalases lack canonical peroxisomal targeting signals (PTS), and the mechanism underlying their PEX5-dependent peroxisomal import remains poorly understood. Here, we expressed CAT2 with N- and C-terminal GFP fusions in an Arabidopsis cat2 mutant background and found that only GFP-CAT2 completely restored wild-type function despite comparable catalase activity for both CAT2-GFP and GFP-CAT2. Only GFP-CAT2 localized to peroxisomes, indicating that altered subcellular localization prevented CAT2-GFP functional complementation. Expression of CAT2-GFP in wild-type Arabidopsis and Nicotiana benthamiana revealed in vivo interaction with endogenous catalases leading to co-import into peroxisomes. Localization of YFP fused to various CAT2 C-terminal sections identified the CAT2 C-terminal five amino acids as a functional PTS1-like sequence. These findings define a minimal C-terminal targeting motif for plant catalases that is sufficient for their peroxisomal import.",
  "abstract_shown": true,
  "affiliations": [
    "University of Geneva"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "name": "Z. Hu"
    },
    {
      "name": "L. Tremulot"
    },
    {
      "name": "S. C. Stolze"
    },
    {
      "name": "H. Nakagami"
    },
    {
      "name": "M. Hothorn"
    },
    {
      "affs": [
        0
      ],
      "name": "R. Ulm"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/0sz8v4m8fqnpe.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.10.10.758186",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.10.10.758186v1.full",
  "id": "0sz8v4m8fqnpe",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.10.10.758186v1",
  "language": null,
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "plant biology"
    ],
    [
      "openalex",
      "1312|Redox biology and oxidative stress"
    ]
  ],
  "openalex": "W7221622272",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.10.10.758186v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-10",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/0sz8v4m8fqnpe.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:27:32Z",
      "native_id": "10.64898/2026.10.10.758186",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.10.10.758186v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:plant biology",
      "subfield": "plant-biology"
    }
  ],
  "title": "Catalase import into peroxisomes in Arabidopsis",
  "title_text": "Catalase import into peroxisomes in Arabidopsis",
  "type": "preprint",
  "updated_at": "2026-10-30T12:27:32Z",
  "url": "https://research.draconitic.com/paper/0sz8v4m8fqnpe",
  "venue": "bioRxiv",
  "versions": []
}