{
  "abstract": "Chronic tympanic membrane (TM) perforations remain a significant clinical problem due to limited spontaneous healing and the drawbacks of surgical interventions. The present study aimed to evaluate the effects of low-intensity infrared laser irradiation on cellular mechanisms of regeneration and to assess its therapeutic potential in TM repair. Primary fibroblast cultures isolated from chinchilla tissue were exposed to laser radiation at wavelengths 1560 nm and 1450 nm. Cellular responses were analyzed using MTT assay, scratch test, Ki-67 immunostaining, and ELISA for collagen type II. In addition, an in vivo model of chronic TM perforation in chinchillas was used to validate the regenerative effects under physiological conditions. Laser irradiation was associated with accelerated scratch closure and increased extracellular matrix production under selected irradiation conditions, whereas metabolic and proliferative indices decreased over time, most likely reflecting culture confluence and maturation rather than cytotoxicity. In vivo, complete closure of tympanic membrane perforations was observed in all laser-treated ears in the context of collagen sponge-assisted repair, whereas scaffold-only control perforations remained unclosed. These findings support the potential of low-intensity infrared laser irradiation as a minimally invasive adjunct to scaffold-assisted tympanic membrane repair. However, the proposed involvement of endogenous progenitor cell populations should be regarded as a hypothesis requiring further histological and marker-based validation.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Clinical Pharmacology and Propaedeutics of Internal Diseases, Sechenov First Moscow State Medical University, Moscow 119991, Russia",
    "Engineering Physics Institute for Biomedicine, Moscow Engineering Physics Institute (National Research Nuclear University), Moscow 115409, Russia",
    "Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology, Laboratory of Pharmacology and Health Technology Assessment, Moscow 117198, Russia",
    "Department of Ear, Nose and Throat Diseases, Sechenov First Moscow State Medical University, Moscow 119991, Russia",
    "National Research Centre “Kurchatov Institute”, Moscow 123182, Russia"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 13,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Ekaterina Valerievna Blinova"
    },
    {
      "affs": [
        1
      ],
      "name": "Igor Vyacheslavovich Lunyov"
    },
    {
      "affs": [
        1,
        2
      ],
      "name": "Andrey Igorevich Osipyants",
      "orcid": "0000-0001-6492-5457"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Kirill Dmitrievich Blinov"
    },
    {
      "affs": [
        3
      ],
      "name": "Anna Vladimirovna Zolotova"
    },
    {
      "affs": [
        3
      ],
      "name": "Liudmila Mikhailovna Ignatova"
    },
    {
      "affs": [
        3
      ],
      "name": "Valeriy Michailovich Svistushkin"
    },
    {
      "affs": [
        3
      ],
      "name": "Valeria Vadimovna Gainutdinova"
    },
    {
      "affs": [
        3
      ],
      "name": "Mikhail Valerievich Svistushkin",
      "orcid": "0000-0002-8552-1395"
    },
    {
      "affs": [
        4
      ],
      "name": "Ekaterina Mikhailovna Kasianenko",
      "orcid": "0000-0002-9380-1821"
    },
    {
      "affs": [
        4
      ],
      "name": "Alexander Ivanovich Omelchenko"
    },
    {
      "affs": [
        4
      ],
      "name": "Aleksey Vasilevich Yuzhakov",
      "orcid": "0000-0002-6555-1925"
    },
    {
      "affs": [
        4
      ],
      "name": "Olga Igorevna Baum"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/0vd11z31zdy6q.bib",
  "cited_by": 0,
  "doi": "10.3390/ijms27198906",
  "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13666236/?tool=EBI",
  "id": "0vd11z31zdy6q",
  "issns": [
    "1422-0067"
  ],
  "landing_url": "https://europepmc.org/article/PMC/PMC13666236",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2741|Laser Applications in Dentistry and Medicine"
    ]
  ],
  "openalex": "W7220844091",
  "pdf_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13666236/pdf/?tool=EBI",
  "pmcid": "PMC13666236",
  "pmid": null,
  "published": "2026-10-01",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/0vd11z31zdy6q.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:31Z",
      "native_id": "PMC:PMC13666236",
      "source": "europepmc",
      "url": "https://europepmc.org/article/PMC/PMC13666236"
    },
    {
      "first_seen": "2026-10-30T12:18:55Z",
      "native_id": "10.3390/ijms27198906",
      "source": "crossref",
      "url": "https://www.mdpi.com/1422-0067/27/19/8906"
    }
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  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2741|Laser Applications in Dentistry and Medicine",
      "subfield": "imaging"
    }
  ],
  "title": "Cellular and Tissue Mechanisms of Laser-Assisted Regeneration of Tympanic Membrane Perforation",
  "title_text": "Cellular and Tissue Mechanisms of Laser-Assisted Regeneration of Tympanic Membrane Perforation",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:18:55Z",
  "url": "https://research.draconitic.com/paper/0vd11z31zdy6q",
  "venue": "International journal of molecular sciences",
  "versions": []
}