{
  "abstract": "Riemerella anatipestifer (RA) is a major cause of bacterial meningitis, which poses a serious threat to the poultry industry. To identify RA strains with a strong ability to cross the blood-brain barrier (BBB) and analyze their genomic characteristics, a co-infection model was established using multiple RA strains with different serotypes in this study. We found that during co-infection with multiple RA strains, certain RA strains rapidly penetrated the BBB and were recovered from brain tissue as early as 0.25 h post-infection. The pioneer strains were selected to further evaluate their BBB-penetrating capacity and pathogenicity in vivo and in vitro. Whole-genome sequencing of three pioneer strains and one RA strain that couldn't penetrate the BBB revealed that these strains had circular chromosomes of 2.14-2.28 Mb, encoding 1,982-2,137 protein-coding genes. All strains carried 40 tRNA genes and 9 rRNA genes, and contained 9-15 genomic islands. Comparative genomic analysis revealed variation in orthogroup composition among the four strains, with strain-specific distributions of resistance genes (24-29 per strain) and virulence genes (4-57 strain-specific genes), alongside variable transposon and IS element profiles. Comparative genomic analysis further uncovered strain-specific distributions of virulence and antibiotic resistance genes, underscoring the genomic plasticity among these isolates. In summary, these findings provide a comprehensive phenotypic and genomic characterization of RA strains with strong BBB-penetrating ability, providing a foundation for future mechanistic studies on RA BBB penetration.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, 271017, China; Shandong Provincial Key Laboratory of Zoonoses, Shandong Agricultural University, Tai'an, 271017, China.",
    "Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, 271017, China; Shandong Provincial Key Laboratory of Zoonoses, Shandong Agricultural University, Tai'an, 271017, China. Electronic address: mengyu@sdau.edu.cn."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 9,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Xinran Li"
    },
    {
      "affs": [
        0
      ],
      "name": "Ying Cheng"
    },
    {
      "affs": [
        0
      ],
      "name": "Shiqian Dang"
    },
    {
      "affs": [
        0
      ],
      "name": "Jiawei Wang"
    },
    {
      "affs": [
        0
      ],
      "name": "Kaisu Yang"
    },
    {
      "affs": [
        0
      ],
      "name": "Haonan Guo"
    },
    {
      "affs": [
        0
      ],
      "name": "Ruihua Zhang"
    },
    {
      "affs": [
        0
      ],
      "name": "Shijin Jiang"
    },
    {
      "affs": [
        1
      ],
      "name": "Yu Meng"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/0vs2w2e2zxwwv.bib",
  "cited_by": 0,
  "doi": "10.1016/j.psj.2026.107944",
  "fulltext_url": "https://europepmc.org/articles/PMC13695245",
  "id": "0vs2w2e2zxwwv",
  "issns": [
    "0032-5791",
    "1525-3171"
  ],
  "landing_url": "https://europepmc.org/article/MED/42893651",
  "language": "eng",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2404|Microbial infections and disease research"
    ]
  ],
  "openalex": "W7221439189",
  "pdf_url": "https://europepmc.org/articles/PMC13695245?pdf=render",
  "pmcid": "PMC13695245",
  "pmid": "42893651",
  "published": "2026-10-11",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/0vs2w2e2zxwwv.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:00Z",
      "native_id": "MED:42893651",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42893651"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:2404|Microbial infections and disease research",
      "subfield": "microbiology"
    }
  ],
  "title": "Identification and whole-genome sequencing of blood-brain barrier-penetrating Riemerella anatipestifer isolated from chickens and ducks.",
  "title_text": "Identification and whole-genome sequencing of blood-brain barrier-penetrating Riemerella anatipestifer isolated from chickens and ducks.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:00Z",
  "url": "https://research.draconitic.com/paper/0vs2w2e2zxwwv",
  "venue": "Poultry science",
  "versions": []
}