{
  "abstract": "Understanding phage K. pneumoniae –host interactions is essential for elucidating phage infection mechanisms and developing phage-derived antibacterial strategies. This showed that lipopolysaccharide (LPS) plays an important role in phage vB_KpnS_MK54 adsorption by constructing the isogenic deletion mutants LPKPΔmsbA and LPKPΔlpxB, in which phage adsorption was reduced by 2–3 log compared with the wild-type strain. Biofilms are associated with persistent infections and increased tolerance to antimicrobial treatment, posing a significant clinical challenge. We further characterized the recombinant depolymerase Dep_Zy, which exhibited potent depolymerase and antibiofilm activities, including depolymerase activity at 0.1 μg/mL, 55% inhibition of biofilm formation at 10 μg/mL, and 30% degradation of mature biofilms at 1 μg/mL. Notably, Dep_Zy potentiated the activity of conventional antibiotics, resulting in a 1–3 log reduction in biofilm-associated bacterial counts when combined with kanamycin, gentamicin, or streptomycin. These findings elucidate the molecular basis of phage–host interactions and highlight Dep_Zy as a promising enzymatic strategy for controlling multidrug-resistant Klebsiella pneumoniae through biofilm disruption and antibiotic potentiation.",
  "abstract_shown": true,
  "affiliations": [
    "College of Veterinary Medicine, Sichuan Agricultural University",
    "Key Laboratory of Agricultural Animal Diseases and Veterinary Public Health of Sichuan Province"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Yan Zeng"
    },
    {
      "affs": [
        0
      ],
      "name": "Zhaomin Wu"
    },
    {
      "affs": [
        0
      ],
      "name": "Zhuangkan Yan"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Yin Wang"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Zexiao Yang"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Yixin Huang"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Suizhong Cao"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Xueping Yao"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/0xdhsk1eevmh8.bib",
  "cited_by": 0,
  "doi": "10.3389/fmicb.2026.1963357",
  "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13690813/?tool=EBI",
  "id": "0xdhsk1eevmh8",
  "issns": [
    "1664-302X"
  ],
  "landing_url": "https://www.frontiersin.org/articles/10.3389/fmicb.2026.1963357/full",
  "language": "eng",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2303|Bacteriophages and microbial interactions"
    ]
  ],
  "openalex": "W7221085968",
  "pdf_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13690813/pdf/?tool=EBI",
  "pmcid": "PMC13690813",
  "pmid": null,
  "published": "2026-10-09",
  "publisher": "Frontiers Media SA",
  "ris": "https://research.draconitic.com/paper/0xdhsk1eevmh8.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:12Z",
      "native_id": "10.3389/fmicb.2026.1963357",
      "source": "crossref",
      "url": "https://www.frontiersin.org/articles/10.3389/fmicb.2026.1963357/full"
    },
    {
      "first_seen": "2026-10-30T12:19:59Z",
      "native_id": "PMC:PMC13690813",
      "source": "europepmc",
      "url": "https://europepmc.org/article/PMC/PMC13690813"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:2303|Bacteriophages and microbial interactions",
      "subfield": "ecology"
    }
  ],
  "title": "Phage vB_KpnS_MK54: lPS-associated factors involved in phage adsorption and depolymerase-driven anti-biofilm effects",
  "title_text": "Phage vB_KpnS_MK54: lPS-associated factors involved in phage adsorption and depolymerase-driven anti-biofilm effects",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:59Z",
  "url": "https://research.draconitic.com/paper/0xdhsk1eevmh8",
  "venue": "Frontiers in Microbiology",
  "versions": []
}