{
  "abstract": "CRISPR/Cas9-mediated homology-directed precise genome editing using long single-stranded DNA (ssDNA) donors has expanded the possibilities for generating defined genetic modifications. However, the required ssDNA donor preparation can be technically demanding and often requires extensive locus-specific sequence design. Here, we examined parameters influencing ssDNA donor-mediated genome editing and developed approaches to simplify donor preparation using {lambda}-exonuclease-mediated ssDNA generation. By evaluating donor designs across multiple genomic loci, we found that efficient genome editing can be achieved with relatively short homology regions for a range of insertion sizes. These findings provide a basis for simplifying ssDNA donor preparation and the overall gene-editing pipeline, potentially facilitating its application across species.",
  "abstract_shown": true,
  "affiliations": [
    "National Centre for Biological Sciences"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "name": "A. Vats"
    },
    {
      "name": "A. Sen"
    },
    {
      "name": "A. Bandyopadhyay"
    },
    {
      "name": "M. Sudhanand"
    },
    {
      "name": "M. X. Mascarenhas"
    },
    {
      "affs": [
        0
      ],
      "name": "A. Bhattacharya"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/0y6g28pvzvc1e.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.09.04.749330",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.09.04.749330v1.full",
  "id": "0y6g28pvzvc1e",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.09.04.749330v1",
  "language": null,
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "molecular biology"
    ],
    [
      "openalex",
      "1312|CRISPR and Genetic Engineering"
    ]
  ],
  "openalex": "W7211880822",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.09.04.749330v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-04",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/0y6g28pvzvc1e.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:58Z",
      "native_id": "10.64898/2026.09.04.749330",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.09.04.749330v1"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "biorxiv:molecular biology",
      "subfield": "molecular-biology"
    }
  ],
  "title": "An Improved Protocol for High-Efficiency and Cost-Effective CRISPR/Cas9-Mediated Knock-ins in C. elegans",
  "title_text": "An Improved Protocol for High-Efficiency and Cost-Effective CRISPR/Cas9-Mediated Knock-ins in C. elegans",
  "type": "preprint",
  "updated_at": "2026-10-30T12:19:58Z",
  "url": "https://research.draconitic.com/paper/0y6g28pvzvc1e",
  "venue": "bioRxiv",
  "versions": []
}