{
  "abstract": "Kidney disease (KD) is a major health problem, and metabolic disorders contribute to diabetic nephropathy and chronic kidney disease, in which renal epithelial cell dysfunction plays an important role. Intracellular advanced glycation end-products (AGEs) may contribute to KD progression by disrupting cellular homeostasis; however, the underlying mechanisms remain unclear. Therefore, we aimed to investigate the effects of glyceraldehyde (GA) on molecular chaperone- and autophagy-related proteins in human renal epithelial cells (HK-2), with particular attention to AGE-related processes. Administration of high-fructose corn syrup to healthy Wistar rats increased argpyrimidine immunoreactivity in the renal tubular epithelial cells, compared with that in controls, suggesting enhanced accumulation of this GA-related AGE in vivo. In HK-2 cells, GA treatment caused concentration-dependent reductions in NADPH and ATP levels, accompanied by morphological changes. High molecular weight (HMW) immunoreactive species of HSP90β, Beclin-1, and ATG7 appeared following GA treatment. GA at 1.5 mM increased LC3-I, LC3-II, and p62 levels, whereas treatment with 2 mM GA attenuated these increases. Aminoguanidine, an inhibitor of AGE formation, attenuated these GA-induced alterations. Overall, GA may induce the generation of HMW species of HSP90β and autophagy-related proteins and may contribute to altered cellular homeostasis, with AGE-related processes potentially contributing to these effects.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Pathology and Laboratory Medicine, Kanazawa Medical University, Uchinada 920-0293, Ishikawa, Japan",
    "Department of Pathology, Kanazawa Medical University Hospital, Uchinada 920-0293, Ishikawa, Japan",
    "Department of Pathophysiological and Experimental Pathology, Kanazawa Medical University, Uchinada 920-0293, Ishikawa, Japan",
    "Department of Pharmacy, Kanazawa Medical University Hospital, Uchinada 920-0293, Ishikawa, Japan",
    "Department of Urology, Kanazawa Medical University, Uchinada 920-0293, Ishikawa, Japan",
    "Department of Oncogenic Pathology, Kanazawa Medical University, Uchinada 920-0293, Ishikawa, Japan",
    "Department of Oral and Maxillofacial Surgery, Kanazawa Medical University, Uchinada 920-0293, Ishikawa, Japan"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 16,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Thao Thanh Nguyen",
      "orcid": "0009-0009-6782-7479"
    },
    {
      "affs": [
        2,
        3
      ],
      "name": "Takanobu Takata",
      "orcid": "0000-0003-0514-8584"
    },
    {
      "affs": [
        4
      ],
      "name": "Shinya Inoue",
      "orcid": "0000-0001-8639-6955"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Dung Anh Vu",
      "orcid": "0009-0007-4427-3739"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Akihiro Shioya",
      "orcid": "0000-0002-1863-5368"
    },
    {
      "affs": [
        2
      ],
      "name": "Miyako Shimasaki",
      "orcid": "0009-0001-0900-5938"
    },
    {
      "affs": [
        2
      ],
      "name": "Motona Kumagai",
      "orcid": "0009-0007-8979-3981"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Jia Han"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Yao Liu"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Wenpeng Jiao"
    },
    {
      "affs": [
        0,
        1,
        5
      ],
      "name": "Mitsuaki Yoshida"
    },
    {
      "affs": [
        6
      ],
      "name": "Yasuhisa Sawai",
      "orcid": "0009-0005-2428-3417"
    },
    {
      "affs": [
        6
      ],
      "name": "Hiroyuki Nakano",
      "orcid": "0000-0002-0532-1853"
    },
    {
      "affs": [
        3
      ],
      "name": "Togen Masauji",
      "orcid": "0009-0000-5897-8800"
    },
    {
      "affs": [
        4
      ],
      "name": "Katsuhito Miyazawa",
      "orcid": "0000-0002-0331-3753"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Sohsuke Yamada",
      "orcid": "0000-0003-2662-0024"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/0yp78q63jvqmr.bib",
  "cited_by": 0,
  "doi": "10.3390/biom16101488",
  "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13702707/?tool=EBI",
  "id": "0yp78q63jvqmr",
  "issns": [
    "2218-273X"
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  "landing_url": "https://www.mdpi.com/2218-273X/16/10/1488",
  "language": "eng",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
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      "1308|Advanced Glycation End Products research"
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  "pdf_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13702707/pdf/?tool=EBI",
  "pmcid": "PMC13702707",
  "pmid": null,
  "published": "2026-10-01",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/0yp78q63jvqmr.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:59Z",
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  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:1308|Advanced Glycation End Products research",
      "subfield": "general"
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  ],
  "title": "Glyceraldehyde May Induce the Generation of High-Molecular-Weight Species of HSP90β and Autophagy-Related Proteins and Alter Protein Levels in Human Renal Epithelial Cells",
  "title_text": "Glyceraldehyde May Induce the Generation of High-Molecular-Weight Species of HSP90β and Autophagy-Related Proteins and Alter Protein Levels in Human Renal Epithelial Cells",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:21:25Z",
  "url": "https://research.draconitic.com/paper/0yp78q63jvqmr",
  "venue": "Biomolecules",
  "versions": []
}