{
  "abstract": "Very rare cells often have outsized physiologic functions. However, their low abundance presents a technical challenge for studying their function. Here, we generate a murine model, which enables the profiling of secreted proteomes (secretomes) from physiologically relevant low-abundance cells in complex tissues, without the use of viral vectors. We then deploy this model to profile the secretomes of two rare innate immune cell types: Kupffer macrophages from whole liver and the rare airway microfold (M) cells in cultures of differentiated airway epithelia. The method we have developed is characterized by remarkable sensitivity, capturing cell-specific proteins that were not captured in single-cell RNA sequencing (scRNA-seq) atlases. We have made this genetic secretome mouse model available to the community to empower future studies of cell-to-cell communication, secreted protein function, and biomarker discovery for any given cell type in vitro or in vivo.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Internal Medicine, Division of Pulmonary and Critical Care, Simches 4, Northeast Corridor, Massachusetts General Hospital, Boston, MA 02114, USA",
    "Department of Pathology, Stanford University School of Medicine, Stanford, CA 94305, USA",
    "Sarafan ChEM-H, Stanford University, Stanford, CA 94305, USA",
    "Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA",
    "Department of Developmental, Molecular and Chemical Biology, School of Medicine, Tufts University, Boston, MA 02111, USA",
    "Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA",
    "The Phil & Penny Knight Initiative for Brain Resilience at the Wu Tsai Neurosciences Institute, Stanford University, Stanford, CA 94305, USA"
  ],
  "article_type": "brief report",
  "arxiv": null,
  "author_total": 11,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Gergana Shipkovenska"
    },
    {
      "affs": [
        1,
        2
      ],
      "name": "Xuchao Lyu"
    },
    {
      "affs": [
        0
      ],
      "name": "Manalee Vishnu Surve"
    },
    {
      "affs": [
        3
      ],
      "name": "Joao A. Paulo"
    },
    {
      "affs": [
        0
      ],
      "name": "Edward Chen"
    },
    {
      "affs": [
        0
      ],
      "name": "Vanshika Panchal"
    },
    {
      "affs": [
        4
      ],
      "name": "Brian Lin"
    },
    {
      "affs": [
        3
      ],
      "name": "Steven P. Gygi"
    },
    {
      "affs": [
        5
      ],
      "name": "Wei Wei"
    },
    {
      "affs": [
        1,
        2,
        6
      ],
      "name": "Jonathan Z. Long"
    },
    {
      "affs": [
        0
      ],
      "name": "Jayaraj Rajagopal"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/0zsthdhwt7z7c.bib",
  "cited_by": 0,
  "doi": "10.1016/j.crmeth.2026.101552",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13721748/",
  "id": "0zsthdhwt7z7c",
  "issns": [
    "2667-2375"
  ],
  "landing_url": "https://doi.org/10.1016/j.crmeth.2026.101552",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Report"
    ],
    [
      "openalex",
      "1607|Advanced Proteomics Techniques and Applications"
    ]
  ],
  "openalex": "W7203464538",
  "pdf_url": null,
  "pmcid": "PMC13721748",
  "pmid": "42600616",
  "published": "2026-08-14",
  "publisher": "Elsevier",
  "ris": "https://research.draconitic.com/paper/0zsthdhwt7z7c.ris",
  "sources": [
    {
      "first_seen": "2026-11-03T12:22:23Z",
      "native_id": "PMC13721748",
      "source": "pmc",
      "url": "https://doi.org/10.1016/j.crmeth.2026.101552"
    }
  ],
  "subjects": [
    {
      "field": "chemistry",
      "origin": "openalex:1607|Advanced Proteomics Techniques and Applications",
      "subfield": "physical"
    }
  ],
  "title": "A sensitive method for profiling cell-type-specific secretomes of rare cells from complex tissues",
  "title_text": "A sensitive method for profiling cell-type-specific secretomes of rare cells from complex tissues",
  "type": "peer-reviewed",
  "updated_at": "2026-11-03T12:22:23Z",
  "url": "https://research.draconitic.com/paper/0zsthdhwt7z7c",
  "venue": "Cell Reports Methods",
  "versions": []
}