{
  "abstract": "Therapeutic oligonucleotides (ONs) have rapidly emerged as a novel class of treatments in the pharmaceutical industry, with over 25 medicines approved in the past decade. Ion-pair reversed-phase liquid chromatography coupled with mass spectrometry (IP-RPLC-MS) is widely used method for ON characterization. This review critically examines the state-of-the-art developments in IP-RPLC-MS for ON analysis, with particular focus on the selection of optimal chromatographic conditions for three key applications: impurity profiling for single-stranded ON, characterization of novel conjugates (e.g., lipid-and peptide-conjugated ONs) and double-stranded siRNA analysis in non-denaturing conditions. Finally, recent developments in ion-pair-free RPLC are discussed in the context of pharmaceutical and bioanalytical applications.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Chemical Engineering Vrije Universiteit Brussel (VUB) Brussels Belgium.",
    "Faculty of Chemistry Department of Analytical Chemistry Gdańsk University of Technology Gdańsk Poland.",
    "Therapeutics Development & Supply Janssen Research & Development NV Johnson & Johnson Company Beerse Belgium.",
    "Waters Corporation Global Research Milford Massachusetts USA."
  ],
  "article_type": "review article",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Quang-Dong Bui",
      "orcid": "0000-0003-4807-727X"
    },
    {
      "affs": [
        1
      ],
      "name": "Weronika Hewelt-Belka"
    },
    {
      "affs": [
        2
      ],
      "name": "Tiny Deschrijver"
    },
    {
      "affs": [
        3
      ],
      "name": "Martin Gilar",
      "orcid": "0000-0003-1199-1287"
    },
    {
      "affs": [
        0
      ],
      "name": "Sebastiaan Eeltink",
      "orcid": "0000-0002-5465-0127"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/10jszvhretp44.bib",
  "cited_by": 0,
  "doi": "10.1002/ansa.70113",
  "fulltext_url": "https://europepmc.org/articles/PMC13710897",
  "id": "10jszvhretp44",
  "issns": [
    "2628-5452"
  ],
  "landing_url": "https://europepmc.org/article/MED/42948273",
  "language": "eng",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Review"
    ],
    [
      "openalex",
      "1602|Analytical Methods in Pharmaceuticals"
    ]
  ],
  "openalex": "W7224024349",
  "pdf_url": "https://europepmc.org/articles/PMC13710897?pdf=render",
  "pmcid": "PMC13710897",
  "pmid": "42948273",
  "published": "2026-10-26",
  "publisher": "Wiley",
  "ris": "https://research.draconitic.com/paper/10jszvhretp44.ris",
  "sources": [
    {
      "first_seen": "2026-10-31T00:20:34Z",
      "native_id": "MED:42948273",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42948273"
    },
    {
      "first_seen": "2026-10-31T12:21:46Z",
      "native_id": "PMC13710897",
      "source": "pmc",
      "url": "https://doi.org/10.1002/ansa.70113"
    }
  ],
  "subjects": [
    {
      "field": "chemistry",
      "origin": "openalex:1602|Analytical Methods in Pharmaceuticals",
      "subfield": "analytical"
    }
  ],
  "title": "Recent Advances in Reversed-Phase Liquid Chromatography for Therapeutic Oligonucleotide and Conjugate Characterization: From Ion-Pairing to Green Analytical Approaches.",
  "title_text": "Recent Advances in Reversed-Phase Liquid Chromatography for Therapeutic Oligonucleotide and Conjugate Characterization: From Ion-Pairing to Green Analytical Approaches.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-31T12:21:46Z",
  "url": "https://research.draconitic.com/paper/10jszvhretp44",
  "venue": "Analytical science advances",
  "versions": []
}