{
  "abstract": "Fibroblast activation protein α (FAP) is a member of the plasma membrane-bound serine protease family, which exhibits exopeptidase and endopeptidase activity in organisms. Recently, considerable studies have focused on the key role of FAP in various pathological processes, indicating that it may be a therapeutic target for treating human disorders and diseases. In the present study, a fluorescent probe for FAP was constructed by the introduction of a substrate recognition dipeptide that was rationally modified by tert-butoxycarbonyl group to the fluorophore DDAN. The probe DDAF, synthesized from DDAN, exhibited high selectivity and sensitivity for FAP, with a detection limit of 0.71 ng mL⁻¹. DDAF was successfully applied for the rapid screening of potent inhibitors of FAP, and a quinoline derivative was identified that exhibited a strong inhibitory effect on FAP. Subsequently, the in vitro visualization and monitoring of FAP in living cancer cells and tissues were performed using DDAF, indicating the widespread expression of FAP in a variety of tumors. These findings provide a promising tool for discovering potent therapeutic agents targeting FAP and for the real-time visualization and monitoring of FAP in tumors.",
  "abstract_shown": true,
  "affiliations": [
    "Second Affiliated Hospital, Dalian Medical University Dalian 116023 China maxc1978@163.com.",
    "Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University Xuzhou 221004 China.",
    "School of Life Science and Technology, Northwestern Polytechnical University Xi'an 710072 China.",
    "College of Pharmacy, Dalian Medical University Dalian 116044 China ningjing0626@163.com.",
    "Department of Neurosurgery, General Hospital of Northern Theater Command Shenyang China liangguobiao6708@vip.163.com."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 11,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Lei Feng",
      "orcid": "0000-0003-2377-0190"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Hengjing Chen"
    },
    {
      "affs": [
        2
      ],
      "name": "Zhenhao Tian"
    },
    {
      "affs": [
        3
      ],
      "name": "Wenpeng Li"
    },
    {
      "affs": [
        3
      ],
      "name": "Yifei Dang"
    },
    {
      "affs": [
        4
      ],
      "name": "Guobiao Liang"
    },
    {
      "affs": [
        0
      ],
      "name": "Chengjuan Xing"
    },
    {
      "affs": [
        3
      ],
      "name": "Xiaohong Shu"
    },
    {
      "affs": [
        3
      ],
      "name": "Jing Ning",
      "orcid": "0000-0002-8035-6802"
    },
    {
      "affs": [
        4
      ],
      "name": "Fangyu Yang"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Xiaochi Ma",
      "orcid": "0000-0003-4397-537X"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/123a3cfhp5jmm.bib",
  "cited_by": 0,
  "doi": "10.1039/d6ra05595h",
  "fulltext_url": "https://europepmc.org/articles/PMC13686149",
  "id": "123a3cfhp5jmm",
  "issns": [
    "2046-2069"
  ],
  "landing_url": "https://europepmc.org/article/MED/42910326",
  "language": "eng",
  "licence": "CC-BY-NC",
  "licence_label": "CC BY-NC",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1607|Molecular Sensors and Ion Detection"
    ]
  ],
  "openalex": "W7223158712",
  "pdf_url": "https://europepmc.org/articles/PMC13686149?pdf=render",
  "pmcid": "PMC13686149",
  "pmid": "42910326",
  "published": "2026-10-21",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/123a3cfhp5jmm.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:20:10Z",
      "native_id": "MED:42910326",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42910326"
    }
  ],
  "subjects": [
    {
      "field": "chemistry",
      "origin": "openalex:1607|Molecular Sensors and Ion Detection",
      "subfield": "physical"
    }
  ],
  "title": "An activatable fluorescent probe for fibroblast activation protein α and its bioimaging applications.",
  "title_text": "An activatable fluorescent probe for fibroblast activation protein α and its bioimaging applications.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:20:10Z",
  "url": "https://research.draconitic.com/paper/123a3cfhp5jmm",
  "venue": "RSC advances",
  "versions": []
}