{
  "abstract": "Transposons are fundamental genetic elements that have profoundly shaped the architecture of eukaryotic genomes. Yeasts and filamentous fungi have emerged as important chassis organisms for bioingredient production in synthetic biology and metabolic engineering. In this review, we summarise the current understanding and future opportunities in the development of transposon‐based tools for genome engineering in these fungal systems. Fungal inverted terminal repeat (ITR) DNA transposons, as well as long terminal repeat (LTR) and non‐LTR retrotransposons, can accelerate genomic mutagenesis, facilitating the screening of superior genotypes and phenotypes. CRISPR‐associated transposons (CASTs) hold considerable potential for site‐specific integration of large transgenes, bypassing the limitations imposed by low homologous recombination (HR) efficiency in non‐Saccharomyces hosts. Overall, transposon‐based tools represent a valuable and underexplored avenue to accelerate genome engineering and strain development in yeasts and filamentous fungi.",
  "abstract_shown": true,
  "affiliations": [
    "School of Biology and Environmental Science, Faculty of Science Queensland University of Technology Brisbane Queensland Australia",
    "Australian Institute for Bioengineering and Nanotechnology (AIBN) The University of Queensland Brisbane Queensland Australia",
    "Engineering Biology Research Centre Kobe University Nada Kobe Japan",
    "Graduate School of Science, Technology and Innovation Kobe University Nada Kobe Japan",
    "College of Life Sciences, National Engineering Research Center for Efficient Utilisation of Soil and Fertiliser Resources Shandong Agricultural University Tai'an Shandong China",
    "CSIRO Advanced Engineering Biology Future Science Platform Dutton Park Queensland Australia",
    "ARC Centre of Excellence in Synthetic Biology Brisbane Queensland Australia",
    "Department of Chemical Science and Engineering, Faculty of Engineering Kobe University Nada Kobe Japan"
  ],
  "article_type": "review article",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Bingyin Peng"
    },
    {
      "affs": [
        2,
        3
      ],
      "name": "Masahiro Tominaga",
      "orcid": "0000-0001-9691-0428"
    },
    {
      "affs": [
        4
      ],
      "name": "Chengqiang Wang"
    },
    {
      "affs": [
        5,
        6
      ],
      "name": "Robert E. Speight"
    },
    {
      "affs": [
        2,
        3,
        7
      ],
      "name": "Jun Ishii",
      "orcid": "0000-0003-2568-515X"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/1334zpy02hq0y.bib",
  "cited_by": 1,
  "doi": "10.1002/yea.70036",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13728883/",
  "id": "1334zpy02hq0y",
  "issns": [
    "0749-503X",
    "1097-0061"
  ],
  "landing_url": "https://doi.org/10.1002/yea.70036",
  "language": "en",
  "licence": "CC-BY-NC-4.0",
  "licence_label": "CC BY-NC 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "REVIEW ARTICLE"
    ],
    [
      "heading",
      "Review Article"
    ],
    [
      "heading",
      "TechnoYeast"
    ],
    [
      "openalex",
      "1312|Fungal and yeast genetics research"
    ]
  ],
  "openalex": "W7168127231",
  "pdf_url": null,
  "pmcid": "PMC13728883",
  "pmid": "42439777",
  "published": "2026-07-13",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/1334zpy02hq0y.ris",
  "sources": [
    {
      "first_seen": "2026-11-07T12:21:45Z",
      "native_id": "PMC13728883",
      "source": "pmc",
      "url": "https://doi.org/10.1002/yea.70036"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Fungal and yeast genetics research",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Transposons as Tools for Future Genome Engineering in Yeasts and Filamentous Fungi",
  "title_text": "Transposons as Tools for Future Genome Engineering in Yeasts and Filamentous Fungi",
  "type": "peer-reviewed",
  "updated_at": "2026-11-07T12:21:45Z",
  "url": "https://research.draconitic.com/paper/1334zpy02hq0y",
  "venue": "Yeast (Chichester, England)",
  "versions": []
}