{
  "abstract": "The human spermatozoon is among the most specialized cells in the body, yet where its proteins reside, where they come from and how they vary between and within men remain largely unresolved. Here we present the deepest proteomic atlas of human spermatozoa to date, identifying 10,828 proteins across six donors. Abundances correlate well between individuals and are anchored by a conserved core of mitochondrial energy-metabolism proteins, while a smaller fraction varies between donations from the same man. By mechanically separating heads from tails, we assigned thousands of previously unlocalized proteins to a compartment, and by comparing seminal fluid from vasectomized and non-vasectomized men we distinguished sperm- and epididymis-derived proteins from accessory-gland secretions. Using a single-cell analysis workflow we further show that the proteomes of individual spermatozoa can be measured, recovering up to ~1,000 protein groups per cell. In a cohort with total fertilization failure, we find recurrent mitochondrial rather than sperm-egg fusion dysregulation. Together or data provides novel insights into the dynamics of the sperm proteome and male infertility.",
  "abstract_shown": true,
  "affiliations": [
    "University of Copenhagen"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "name": "L. van der Hoeven"
    },
    {
      "name": "L. Schmidt"
    },
    {
      "name": "S. Ziebe"
    },
    {
      "name": "M. R. Petersen"
    },
    {
      "name": "K. Almstrup"
    },
    {
      "name": "T. S. Batth"
    },
    {
      "name": "A. Rehfeld"
    },
    {
      "affs": [
        0
      ],
      "name": "J. V. Olsen"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/162raj2xqyv6k.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.09.22.753411",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.09.22.753411v1.full",
  "id": "162raj2xqyv6k",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.09.22.753411v1",
  "language": null,
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "cell biology"
    ],
    [
      "openalex",
      "2743|Sperm and Testicular Function"
    ]
  ],
  "openalex": "W7214111751",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.09.22.753411v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-22",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/162raj2xqyv6k.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:24:00Z",
      "native_id": "10.64898/2026.09.22.753411",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.09.22.753411v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:cell biology",
      "subfield": "cell-biology"
    }
  ],
  "title": "Mapping the human sperm proteome across compartments, donors and individual cells",
  "title_text": "Mapping the human sperm proteome across compartments, donors and individual cells",
  "type": "preprint",
  "updated_at": "2026-10-30T12:24:00Z",
  "url": "https://research.draconitic.com/paper/162raj2xqyv6k",
  "venue": "bioRxiv",
  "versions": []
}