{
  "abstract": "GeneCapture is developing a novel, point-of-care, rapid AST technique that, when fully automated, can accomplish AST within 2 hours directly from a raw sample, representing a significant advancement in diagnostic capabilities. For this study, a panel of uropathogen isolates was collected from clinical specimens positive for urinary tract infection from various laboratories in the southern US. A selection of these isolates were then spiked into fresh clean-catch urine samples to produce mock samples covering a variety of antibiotic responses. Testing was performed on 20 strains of Escherichia coli, the cause of over 80% of UTIs, and 6 strains each of Klebsiella pneumoniae and Proteus mirabilis, the next most common causes. Each strain was tested against the susceptible, intermediate and resistant (S-I-R) breakpoints of up to 5 appropriate antibiotics including Amoxicillin-Clavulanate, Cefpodoxime, Ciprofloxacin, Nitrofurantoin and Trimethoprim-Sulfamethoxazole. The new method uses a phenotypic assessment of bacterial growth, comparing responses from bacteria treated with antibiotics at S-I-R breakpoint concentrations to untreated controls as the metric for determining susceptibility. Exposure to the drugs for only 90 minutes gave a Categorical Agreement of 98% to gold standard culture results across the resulting 247 bacteria/antibiotic pairs. Testing on separate days of nine of these strains in triplicate gave a reproducibility of 97%. The sequential steps of this novel AST - sample processing, growth, labeling, and counting - can readily be performed on an automated cartridge that is currently under development. The presented work, which took place in a laboratory setting, demonstrates the efficacy of the assay.",
  "abstract_shown": true,
  "affiliations": [
    "GeneCapture Inc"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "name": "M. Roegner"
    },
    {
      "name": "C. Ahmadi"
    },
    {
      "name": "S. Lyon"
    },
    {
      "name": "Z. McGee"
    },
    {
      "name": "K. Chittur"
    },
    {
      "affs": [
        0
      ],
      "name": "P. Millirons"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/16j8155df6enw.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.09.21.753156",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.09.21.753156v1.full",
  "id": "16j8155df6enw",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.09.21.753156v1",
  "language": null,
  "licence": "CC-BY-ND",
  "licence_label": "CC BY-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "microbiology"
    ],
    [
      "openalex",
      "1308|Bacterial Identification and Susceptibility Testing"
    ]
  ],
  "openalex": "W7214128347",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.09.21.753156v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-21",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/16j8155df6enw.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:24:09Z",
      "native_id": "10.64898/2026.09.21.753156",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.09.21.753156v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:microbiology",
      "subfield": "microbiology"
    }
  ],
  "title": "Rapid Phenotypic Antibiotic Susceptibility Determination Direct From Spiked Urine Samples Shows Strong Correlation with Gold Standard for Urinary Tract Infection",
  "title_text": "Rapid Phenotypic Antibiotic Susceptibility Determination Direct From Spiked Urine Samples Shows Strong Correlation with Gold Standard for Urinary Tract Infection",
  "type": "preprint",
  "updated_at": "2026-10-30T12:24:09Z",
  "url": "https://research.draconitic.com/paper/16j8155df6enw",
  "venue": "bioRxiv",
  "versions": []
}