{
  "abstract": "Constructing combinatorially complete species assemblages is often necessary to dissect the complexity of microbial interactions and to find optimal microbial consortia. At the moment, this is accomplished through either painstaking, labor intensive liquid handling procedures, or through the use of state-of-the-art microfluidic devices. Here we present a simple, rapid, low-cost, and highly accessible liquid handling methodology for assembling all possible combinations of a library of microbial strains, which can be implemented with basic laboratory equipment. To demonstrate the usefulness of this methodology, we construct a combinatorially complete set of consortia from a library of eight Pseudomonas aeruginosa strains, and empirically measure the community-function landscape of biomass productivity, identify the highest yield community, and dissect the interactions that lead to its optimal function. This easy to implement, inexpensive methodology will make the assembly of combinatorially complete microbial consortia easily accessible for all laboratories.",
  "abstract_shown": true,
  "affiliations": [
    "Centro Nacional de Biotecnologia (CNB), CSIC, 28049 Madrid, Spain",
    "Instituto de Biología Funcional y Genómica (IBFG), CSIC & Universidad de Salamanca, 37007 Salamanca, Spain",
    "Instituto de Productos Lácteos de Asturias (IPLA), CSIC, 33011 Oviedo, Spain",
    "Institute of Functional Biology and Genomics (IBFG-CSIC)",
    "Grupo Interdisciplinar de Sistemas Complejos (GISC), Departamento de Matemáticas, Universidad Carlos III de Madrid, 28911 Leganés, Spain",
    "Universidad Carlos III de Madrid"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0,
        1,
        2,
        3
      ],
      "name": "Juan Díaz‐Colunga",
      "orcid": "0000-0001-8995-4529"
    },
    {
      "affs": [
        4,
        5
      ],
      "name": "Pablo Catalán",
      "orcid": "0000-0003-2826-4684"
    },
    {
      "affs": [
        0,
        1,
        3
      ],
      "name": "Magdalena San Román",
      "orcid": "0000-0002-2593-7727"
    },
    {
      "affs": [
        0,
        1,
        3
      ],
      "name": "Andrea Arrabal",
      "orcid": "0009-0004-6824-8798"
    },
    {
      "affs": [
        0,
        1,
        3
      ],
      "name": "Álvaro Sánchez",
      "orcid": "0000-0002-2292-5608"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/16r63jjje7zfc.bib",
  "cited_by": 11,
  "doi": "10.7554/elife.101906.3",
  "fulltext_url": null,
  "id": "16r63jjje7zfc",
  "issns": [
    "2050-084X"
  ],
  "landing_url": "https://doi.org/10.7554/elife.101906.3",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Microbial Metabolic Engineering and Bioproduction"
    ]
  ],
  "openalex": "W4396656148",
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-05-19",
  "publisher": "eLife Sciences Publications Ltd",
  "ris": "https://research.draconitic.com/paper/16r63jjje7zfc.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T13:26:46Z",
      "native_id": "W4396656148",
      "source": "openalex",
      "url": "https://doi.org/10.7554/elife.101906.3"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Microbial Metabolic Engineering and Bioproduction",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Full factorial construction of synthetic microbial communities",
  "title_text": "Full factorial construction of synthetic microbial communities",
  "type": "peer-reviewed",
  "updated_at": "2026-11-09T13:21:07Z",
  "url": "https://research.draconitic.com/paper/16r63jjje7zfc",
  "venue": "eLife",
  "versions": []
}